申克孢子丝菌的扫描电镜观察  

Microscopic observation of the Sporothrix schenckii with the scanning electron microscope

作　　者：黄怀球[1] 袁立燕[1] 张静[2] 钟毅[1] 赵静[1] 张晓辉[3] 薛汝增[3] 李美荣[1] 陈荣章[1] 李文娟[1] 

机构地区：[1]中山大学附属第三医院,广东广州510630 [2]中山大学孙逸仙纪念医院,广东广州510020 [3]广东省皮肤性病防治中心,广东广州510500

出　　处：《热带医学杂志》2012年第12期1442-1443,共2页Journal of Tropical Medicine

基　　金：广东省自然科学基金(S2012020011056);广东省科技计划项目(2011B031800277)

摘　　要：目的观察耐伊曲康唑申克孢子丝菌的超微结构特征,为申克孢子丝菌的临床鉴定提供基础资料。方法使用玻片法培养申克孢子丝菌,处理后在电镜下观察、拍照及测量。结果孢子丝菌标本图像清晰立体,可见假轴状分生孢子梗,分生孢子通常为孤立、直立生长,圆锥顶端相对,沿着菌丝轴增殖扩散,形成群集的细齿状的分生孢子链,分生孢子链较长,可弯曲。分生孢子为卵形,细长,约2.0μm×3.5μm大小,透明,表面不光滑,有不规则凹陷。结论该方法准确、可靠,可为申克孢子丝菌临床鉴定提供理论依据。Objective To evaluate the application of using scanning electron microscope in the clinical diagnosis of an itraconazole-resistant Sporothrix schenckii. Methods Sporothrix schenckii was cultivated with microculturing method and the external morphology was analyzed using a scanning electron microscope. Results A clear three-dimensional image can be obtained under the scanning electron microscope. conidia reach out at right angle from the thin menbrane of the hyphae , most of which is isolated and vertical, with the cones stretch opposite to each other. Conidia spread along the hyphae axis, forming clusters of gear-like conidia chains, sometimes appear to be sleeve-shaped, sometimes quincuncial, part of which bend or fracture. Conidia are ovoid, about 2.0 μm×3.5 μm the size, transparent, with the surface unsmooth and irregular pits. Conclusion The obtained images can be used in the identification of Sporothrix schenckii.

关 键 词：申克孢子丝菌 扫描电镜 

分 类 号：R379.9[医药卫生—病原生物学]