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机构地区:[1]新疆医科大学附属肿瘤医院病理科,新疆维吾尔自治区乌鲁木齐市830011 [2]北京大学医学部病理系,北京市100191
出 处:《世界华人消化杂志》2012年第33期3218-3224,共7页World Chinese Journal of Digestology
摘 要:目的:探讨错配修复基因hMLH-1和hMSH-2蛋白在新疆汉族散发性大肠癌(sporadic colorectal carcinoma,SCC)中的表达情况及其与SCC病理参数的关系.方法:采用免疫组织化学方法检测95例汉族SCC组织和65例正常结直肠组织(normal colorectal mucosa,NCM)中hMLH-1和hMSH-2蛋白的表达情况.结果:hMLH-1和hMSH-2在NCM中均表达.hMLH-1蛋白在SCC组织中缺失率为(31.6%)与NCM比较有显著差异(P<0.05);hMSH-2蛋白在SCC组织中缺失率(18.9%)与NCM比较有显著差异(P<0.05).hMLH-1在右半结肠癌中缺失率高于左半结肠癌和直肠癌(P=0.006),在低分化腺癌和黏液腺癌中缺失率明显高于高-中分化腺癌(P=0.014);hMSH-2蛋白在右半结肠癌中缺失率高于左半结肠癌和直肠癌(P=0.002),低分化腺癌和黏液腺癌中缺失率显著高于高-中分化腺癌(P=0.008).在SCC组织中,hMLH-1和hMSH-2蛋白表达呈正相关性(r=0.224,P<0.05).结论:hMLH-1和hMSH-2蛋白在新疆地区汉族散发性大肠癌中有一定比例的缺失,且与肿瘤分化程度和发生部位密切相关,hMLH-1和hMSH-2蛋白联合检测可能为判断散发性直肠癌恶性程度和临床预测提供重要参考.AIM: To investigate the clinical significance of expression of hMLH-1 and hMSH-2 in sporadic colorectal carcinoma (SCC) in Chinese Han pa- tients in Xinjiang. METHODS: Expression of hMLH-1 and hMSH-2 was detected by immunohistochemistry in 95 cases of SCC specimens collected from Chinese Han patients in Xinjiang and 65 cases of normal colorectal mucosa (NCM) specimens. RESULTS: The positive rates of hMLH-1 andhMSH-2 expression in NCM were both 100%. The negative rates of hMLH-1 (31.6%) and hMSH-2 (18.9%) expression were significantly higher in SCC than in NCM (both P 〈 0.05). The negative rates of hMLH-1 and hMSH-2 protein expression were significantly higher in right-sid- ed tumors than in left-sided and rectal tumors (P = 0.006, 0.002), and in poorly differentiated tumors than in well differentiated tumors (P = 0.014, 0.008). There was a positive correlation between the expression of hMLH-1 and hMSH-2 proteins in SCC. CONCLUSION: Loss of expression of hMLH-1 and hMSH-2 proteins in SCC was observed in some Chinese Han patients in Xinjiang. Loss of expression of hMLH-1 and hMSH-2 proteins cor- relates with tumor differentiation and location. Combined detection of hMLH-1 and hMSH-2 expression may be used for evaluating the sever- ity and prognosis of colorectal cancer.
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