实验性变态反应性脑脊髓炎促进大鼠正中隆起细胞凋亡  

Experimental allergic encephalomyelitis promotes cell apoptosis in the median eminence of rat

作　　者：刘梅[1] 曹翠丽[2] 马常升[2] 王丽梅[1] 

机构地区：[1]河北医科大学 西山校区外科教研室,石家庄050200 [2]河北医科大学 基础医学研究所神经生物学研究室,石家庄050017

出　　处：《解剖学杂志》2012年第6期777-780,共4页Chinese Journal of Anatomy

基　　金：河北省自然科学基金（302491,C2005000724）

摘　　要：目的：观察实验性变态反应性脑脊髓炎（EAE）大鼠发病不同时期正中隆起凋亡细胞的变化，探讨该室周器官在EAE病程中的意义。方法：以豚鼠脊髓匀浆和完全弗氏佐剂及百日咳杆菌制备免疫抗原，建立大鼠EAE模型，利用TUNEL技术检测大鼠正中隆起及第三脑室侧壁在EAE不同时期凋亡细胞的变化。结果：接种后不同时期正中隆起和第三脑室侧壁均可见凋亡细胞，且14d组〉7d组〉21d组〉对照组；除对照组外，其他各组正中隆起的凋亡细胞数均多于第三脑室侧壁。结论：在EAE不同时期正中隆起的凋亡细胞变化比第三脑室侧壁突出，与临床病情呈一致性，正中隆起是脑内免疫反应敏感部位。Objeetive： To observe the changes of apoptotic cells in the median eminence of rats in different periods of experimental allergic encephalomyelitis, and to discuss the potential role of the median eminence in EAE. Methods： Rats were actively immunized with guinea pig spinal cord homogenate, complete freund adjuvant and pertussis bacillus emulsions to induce EAE. TUNEL was used to detect apoptotic cells in the median eminence and the adjacent lateral wall of the third ventricle. Changes of apoptotic cells were compared between groups. Results： Apoptotic cells can be found in the median eminence and the lateral wall of the third ventricle in different post inoculation periods of EAE. The trend of number of apoptosis cells was 14 d group〉 7 d group〉21 d group〉control group. The number of apoptosis cells in the median eminence was higher than that in the lateral wall of the third ventricle in different period groups besides the control group. Conclusion： The changes of apoptosis cells in the median eminence were more significant than that in the lateral wall of the third ventricle during EAE and consistent with the pathogenetic condition, inferring that median eminence may be a sensitive site to blood borne immunomessages in the brain.

关 键 词：实验性变态反应性脑脊髓炎 正中隆起 第三脑室 凋亡细胞 大鼠 

分 类 号：R744.3[医药卫生—神经病学与精神病学]