骨髓基质干细胞对腺样囊性癌细胞培养液趋化性的实验研究  

Experimental study of the tropism of bone marrow stromal cells to adenoid cystic carcinoma cells cultural supernatant

作　　者：张凤兰[1] 吴巍[2] 李宗文[2] 苏明[3] 李华[3] 

机构地区：[1]中国食品药品检定研究院食品化妆品检定所,北京100050 [2]首都医科大学基础医学院生物化学与分子生物学教研室 [3]首都医科大学口腔医学院口腔颌面外科

出　　处：《北京口腔医学》2012年第6期322-324,共3页Beijing Journal of Stomatology

基　　金：首都医科大学基础-临床科研合作基金(11JL58)

摘　　要：目的初步探讨骨髓基质干细胞(BMSCs)作为人涎腺腺样囊性癌(SACC)微环境靶向治疗载体的可能性。方法常规培养SACC及T293细胞(阴性对照)至对数生长期,无血清培养48h后收集上清,过滤后置于Transwell小室的下室(即24孔板底部,每孔600-800ul),中间隔以Transwell膜,在上室中加入100～150μl对数生长期的BMSCs无血清悬液,续培24h后取出Transwell膜,固定、染色,观察并计数迁移至Transwell膜表面上的BMSCs细胞,采用t检验进行统计学处理。结果显微镜下随机取5个高倍镜视野,计数各组Transwell膜上迁移的BMSCs细胞,结果显示SACC-LM组为64.3±7.6,SACC-83组为52.3±6.1,均高于阴性对照组(T293)的31.8±6.3,差异具有统计学意义(P<0.05)。结论 BMSCs对人涎腺腺样囊性癌细胞培养上清液具有明显趋化性。BMSCs细胞具有作为腺样囊性癌微环境靶向治疗载体的可能性。Objective To investigate the possibility of bone marrow stromal cells （BMSCs） as a therapy vector targeted to the microenvironment of adenoid cystic carcinoma（ACC）. Methods The ACC and T293 （control） cells were cultured to the logarithmic growth phase, and their serum-free cultural supernatant was collected after cultured for 48 hours. The supernatant was then added to each well of 24-well plate. The transwell membrane was gently placed into the well, and 100-150μl suspension of BMSCs was added into the upper chamber. After cultured for 24 hours, the transwell chamber was removed, fixed and stained. The cells attached to the transwell membrane were observed and counted. The data were statistically analyzed by student t test. Results The migrated cell number of SACC-LM group was 64. 3 ± 7. 6, the SACC-83 group was 52. 3± 6. 1, beth were higher than that of the T293 group（31.8 ± 6. 3）, and the difference between SAAC group and T293 group was significant （p 〈 0. 05 ）. Conclusion Bone marrow stromal ceils had obvious chemntaxis to ACC cultural supernatants and may have the possibility of serving as a targeted therapy vectors for ACC.

关 键 词：腺样囊性癌 骨髓基质干细胞 趋化性 

分 类 号：R739.87[医药卫生—肿瘤]