稻瘟菌的胞外纤维素酶和果胶酶活性的研究  

Activity of cellulase and pectinase in peripery of Piricularia Oryzae Cavara

作　　者：游树鹏[1] 韩献忠[1] 徐礼根[1] 金逸民[1] 

机构地区：[1]杭州大学

出　　处：《安徽农业科学》1991年第4期355-358,共4页Journal of Anhui Agricultural Sciences

摘　　要：液体培养5种稻瘟菌生理小种.随着培养天数增加,ZC_3、ZD_1和ZG_1培养液中纤维素酶活性增高较快,而ZE_1和ZF_1增高较慢.培养液中纤维素酶活性增高快的,葡萄糖含量增加也快.培养8天的稻瘟菌培养液中各生理小种果胶酶活性高低顺序,果胶甲酯酶为:ZD_1>ZC_3>ZG_1>ZE_1>ZF_1;多聚半乳糖醛酸酶活性为:ZD_1>ZC_3>ZE_1>ZF_1>ZG_1.研究表明,胞外纤维素酶和果胶酶活性高的稻瘟菌生理小种,其致病性也强.The Cellulase activity and content of glucose of Piricularia OryzaeCavara in races ZC_3, ZD_1, ZE_1, ZF_1 and ZG_1 in liquid culture were determinedafter different culture days The results were shown as follows: (1) Cellulaseactivity of P. O. Cavara in races ZC_3, ZD_1and ZG_1was higher than that of P. O. Ca-vara in races ZE_1, ZF_1; (2)The content of glucose in culture solution was higherin accordance with higher cellulase activity; (3)Cellulase activity decreased,but the content of glucose increased significantly after 20 days. Pectin methy-lesterase activity of 5 races in liquid cultrue after 8 days was; ZD_1>ZC_3>ZG_1>ZE_1>ZF_1. There were positive correlations between the pathogenicity of P.O. Carara and the activity of cellulase and pectinase.

关 键 词：稻瘟菌 纤维素酶 果胶酶 活性 水稻 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]