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作 者:张新全[1] 颜济[2] 杨俊良[2] 郑有良[2] 伍碧华[2]
机构地区:[1]四川农业大学动物科技学院草业科学系,四川雅安625014 [2]四川农业大学小麦研究所,四川都江堰611830
出 处:《云南植物研究》2000年第2期155-160,共6页Acta Botanica Yunnanica
基 金:国家自然科学基金;四川省教委资助
摘 要:为了探索阿拉善鹅观草RoegneriaalashanicaKeng、大丛鹅观草RoegneriamagnicaespesD .F .Cui与纤毛鹅观草Roegneriaciliaris (Trin .)Nevski间的相互关系 ,将其进行了远缘杂交 ,通过幼胚离体培养 ,两个组合均成功合成了杂种。对亲本及杂种F1 花粉母细胞减数分裂中期Ⅰ染色体配对行为及形态学进行了统计分析。结果表明 ,上述物种的种间杂交较难进行 ,杂种F1 减数分裂染色体平均构型分别为 :R ciliaris×R alashanica 10 6 2Ⅰ +8 17Ⅱ +0 32Ⅲ +0 0 2Ⅳ (c -值 =0 4 4 ) ,R ciliaris×R magnicaespes 18 0 0Ⅰ +4 76Ⅱ +0 16Ⅲ (c -值 =0 2 1) ;杂种穗部特征多数介于双亲之间。阿拉善鹅观草、大丛鹅观草与纤毛鹅观草间至少有一个基因组具有较高的同源性 ,即为S基因组 ,本文对它们在分类中的地位也进行了讨论。In order to investigate the relationships between Roegneria. ciliaris (Trin.) Nevski and R alashanica Keng, and between R ciliaris and R magnicaespes D.F.Cui. R ciliaris (Trin.) Nevski was crossed with the other two species, through the aid of embryo rescue. Hybrids were obtained from these two combinations. Chromosome pairing behavior of parents and hybrids was observed at metaphase I of pollen mother cells. Meiotic configuratons were 10 62Ⅰ+8 17Ⅱ+0 32Ⅲ+0 02Ⅳ for R ciliaris×R alashanica and 18 00Ⅰ+4 76Ⅱ+0 16Ⅲ for R ciliaris×R magnicaespes ; c-value of the two hybrids were 0 44 and 0 21, respectively. It is concluded from the cytological study of F1 hybrids that R magnicaespes and R alashanica at least share one set of S genome of R ciliaris . Most of the hybrid morphology was intermediate between the parents.
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