脑膜瘤内的血管分布和血管生成与HIF-1α、VEGF的关系  

Distribution of blood vessels and relationship of angiogenesis with hypoxia-inducible factor -1αand vascular endothelial growth factor in meningiomas

作　　者：罗勇[1] 林少华[1] 黄权[2] 

机构地区：[1]中山市人民医院神经外科,528400 [2]中山大学附属第一医院神经外科,广州510080

出　　处：《中华神经医学杂志》2013年第1期68-71,共4页Chinese Journal of Neuromedicine

摘　　要：目的 探讨脑膜瘤内部的血管分布以及缺氧诱导因子-1α（HIF-1α）、血管内皮生长因子（VEGF）与脑膜瘤血管生成的关系。方法 选取中山大学附属一院和中山市人民医院神经外科自2010年5月至2011年3月间手术切除并经病理证实的人脑膜瘤标本15例，收集每例标本硬膜鼠尾征处、基底中心、瘤中央、瘤缘4个不同部位的脑膜瘤组织，每个部位分别连续切片3张。应用免疫组化染色检测CD34、VEGF、HIF-1α的分布和表达。结果CD34主要表达在细胞膜和细胞浆，染色呈棕黄色颗粒，ⅦGF定位于肿瘤细胞胞浆内，而HIF-1α主要在细胞核，少量在胞浆。尾征和瘤缘部位MVD、VEGF、HIF-1α的表达高于基底中心和瘤中央，差异有统计学意义（P〈0．05）。脑膜瘤MVD与VEGF、MVD与HIF-1α、VEGF与HIF-1α表达之间均呈正相关关系（r=0．960，P=0．040；r=0．964，P=0．036；r=0．998，P=0．002）。结论脑膜瘤外周部的血管生成比中央区丰富；HIF-1α、VEGF在诱导并促进脑膜瘤的血管生成方面发挥一定作用。Objective To investigate the distribution of blood vessels and the relationship of angiogenesis with hypoxia-inducible factor-let （HIF-1α） and vascular endothelial growth factor （VEGF） in meningiomas. Methods Fifteen specimens ofmeningiomas, collected in our hospital during the resection and conformed by pathology from May 2010 to March 2011, were chosen; 4 different tissues from each specimen were taken, including the tail sign, base center, tumor＇ s center and tumor＇ s margin; three successive slices were made from each tissue part. Envision immunohistochemistry was employed to detect the expressions of CD34, VEGF and HIF-1α and their different distributions were compared. Results CD34 mainly disturbed in the cell membrane and cytoplasm, showing yellow granules in the dyeing; VEGF located at the cytoplasm of the tumor cells, while HIF-1α mainly at the nucleus. The expressions of CD34, VEGF and HIF-1α in the tail sign and tumor＇ s margin were significantly higher than those in the MVD in the base center and tumor＇s center （P〈0.05）. Positive correlations between expressions of VEGF and MVD, expressions of HIF-1α and MVD, as well as expressions of HIF-1α and VEGF were noted （r=0.960, P=0.040; r=0.964, P=-0.036; r=0.998, P=0.002）. Conclusion The angiogenesis in the periphery ofmeningiomas is much richer than that in the center; HIF-1α and VEGF may induce and promote the angiogenesis of meningiomas.

关 键 词：脑膜瘤 微血管密度 血管内皮生长因子 缺氧诱导因子-1Α 

分 类 号：R739.45[医药卫生—肿瘤]