Comparative Study of Immunological Properties on Glutamine Synthetase Isozymes in Rice Plants  

水稻谷氨酰胺合成酶同工酶免疫学性质比较研究(英文)

作　　者：林清华[1] 李常健[1] 张楚富[1] 彭进[1] PENGShao-Bing JohnBENNETT 

机构地区：[1]武汉大学生命科学学院,武汉430072 [2]InternationalRiceResearchInstitute

出　　处：《Acta Botanica Sinica》2000年第5期471-475,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：TheNationalNaturalScienceFoundationofChina!( 3 9870 5 2 7) ;CooperativeFoundationofInternationalRiceResearchInstitute

摘　　要：In rice (Oryza sativa L.) roots two glutamine synthetase (GS) isozymes, GSra and GSrb, were identified recently in the author's experiments, but the homology of both GSra and GSrb as well as their localization in the rice roots are unclear. In the present study, the purified GSra and GSrb from rice roots were used to immunize rabbits to obtain the respective antibodies. The immunodiffusion and immunoblotting experiments showed that the antibody against GSra or GSrb was specific for GS and its isozymes. The immunoprecipitation test indicated that the antibody of GSra or GSrb not only recognized its respective antigen, but also well recognized each other's antigen. GSra or GSrb antibody recognized also better cytosolic GS1 of rice leaves, but the recognization for chloroplast GS2 from rice or spinach (Spinacia oleracea Mill.) leaves was weaker. Our results indicate that GSra and GSrb from rice roots are quite similar in antigenicity and are extremely similar proteins and that both GSra and GSrb may also be a form of cytosolic GS just as the cytosolic GS1 of rice leaves.用纯化的水稻 (OryzasativaL .)根部存在的两种谷氨酰胺合成酶 (GS)同工酶GSra和GSrb分别免疫兔子 ,得到相应的抗体。免疫扩散和免疫印迹实验表明 ,GSra或GSrb的抗体对GS及其同工酶是专一的。免疫沉淀试验表明 ,GSra或GSrb不仅识别它的相应的抗原 ,而且也能很好地识别彼此的抗原。这两种抗体也能较好地识别水稻叶片胞液型的GS1,但对水稻叶片和菠菜 (SpinaciaoleraceaMill.)叶片叶绿体型的GS2的识别较弱。这些结果表明 ,水稻根的GSra和GSrb有很相似的抗原性 ,他们是非常相似的蛋白质。揭示GSra和GSrb像GS1一样都是胞液型GS。

关 键 词：ANTIGEN ANTIBODY glutamine synthetase ISOZYME rice 

分 类 号：Q946.5[生物学—植物学]