固定化嗜热脂肪芽孢杆菌连续合成半乳糖寡糖的研究  

Study on Continuous Synthesis of Galacto oligosaccharide by Immobilized Bacillus stearothermophilus

作　　者：魏东芝[1] 王筱兰 陈少欣[1] 俞俊棠[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室生物化学研究所,上海200237

出　　处：《生物工程学报》2000年第3期392-395,共4页Chinese Journal of Biotechnology

摘　　要：利用固定了产 β 半乳糖苷酶的嗜热脂肪芽孢杆菌 ,以乳糖为底物 ,在纤维床反应器中连续合成半乳糖寡糖 (GOS) ,最高得率为 5 0 7%。在连续反应体系中 ,研究了底物浓度、pH、反应温度和停留时间对半乳糖寡糖合成的影响 ,确定最佳反应条件为底物浓度 45 0 g/L、反应温度 5 5℃、pH7 0、停留时间 10 0min。在连续反应 2 4h后 ,流加 1 5 %的D 半乳糖能提高合成GOS的能力 ,固定化细胞反应体系中连续稳定操作 12 0h。The galacto oligosaccharide was synthesized continuously by immobilized Bacillus stearothermophilu producing β galactosidase in fibrous bed reactor.The effect of substrate concentration,pH,reaction temperature and retention time on production of GOS was investigated.The optimal reaction conditions were determined.Substrate concentration were 450 g/L;Reaction temperature was 55℃;pH was 7.0;Residience time was 100 min.The product yield reached up to 50 7%.GOS synthesis was promoted by feeding 1.5% D glactose after 24h.The immobilized cell reactor can work stably for 120h.

关 键 词：半乳糖寡糖 固定化细胞 纤维床反应器 产率 

分 类 号：Q53[生物学—生物化学]