胃癌弱差异基因表达谱中TINF2基因的表达和生物学作用  

BIOLOGICAL FUNCTION AND SIGNIFICANCE OF LOW VARIANCE EXPRESSION GENE TINF2 IN GASTRIC CANCER GENE EXPRESSION PROFILING

作　　者：孙伟[1] 高芳[2] 王晓龙[2] 龙启福[1] 王嵘[1] 顾存林[1] 沈国平[1] 许博林[1] 耿排力[1] 

机构地区：[1]青海大学医学院基础医学部 [2]青海大学附属医院

出　　处：《青海医学院学报》2012年第4期235-238,共4页Journal of Qinghai Medical College

基　　金：青海大学中青年科研基金(2008-QY-08)

摘　　要：目的明确胃癌差异基因表达谱中弱差异表达基因的敏感性和生物学意义;探讨胃癌弱差异表达基因TINF2在胃癌发生发展中的生物学作用和意义。方法利用GoMiner软件研究TINF2的生物学功能。并利用Real-Time PCR法以及Western blot法验证胃癌弱差异基因表达谱中TINF2基因的表达水平。结果弱差异表达基因TINF2定位于染色体端粒区,主要具有蛋白结合、端粒DNA结合活性,通过端粒酶调节端粒长度的功能;通过20对胃癌及配对的癌旁正常组织实验室验证,TINF2的mRNA及蛋白表达水平在胃癌组织中均表现为低表达(17/20,85%),差异具有显著性(P<0.001),与前期基因芯片中胃癌TINF2表达水平一致。结论 TINF2异常表达与胃癌的发生联系密切,并且可能在胃癌的发生发展中发挥重要的生物学作用。objective To confirm the sensitivity and specificity of the low variance expression gene in gastric cancer gene expression profiling and to understand whether low variance expression gene TINF2 possess important biological functions in development of gastric cancer.Methods The expression of TINF2 gene in gastric cancer tissue was verified by Real-Time PCR and Western blot;Furthermore,the gene function was analyzed by GoMiner software.Results The expression of TINF was slightly lower than that of normal-appearing tissue（fold change1.5）and matched with gene expression profiling in tumor tissue.The GoMiner analysis showed that TINF2 has an important physiologic functions involve in telomeres and has a compacted relation in tumorgenesis.Conclusion The low variant gene of gastric cancer gene expression profiling can be proved;TINF2 may have an important biological role in the development of gastric cancer.

关 键 词：胃癌 基因表达谱 TINF2 

分 类 号：R735.2[医药卫生—肿瘤]