香菇单孢杂交及杂合子鉴定的研究  

Study on Monospore Cross and Identification of Heterozygotes of Shiitake

作　　者：陈世通[1] 白建波[1] 李梦杰[1] 李荣春[1] 

机构地区：[1]云南农业大学食用菌研究所,云南昆明650201

出　　处：《中国食用菌》2013年第1期45-46,55,共3页Edible Fungi of China

摘　　要：以香菇栽培品种"大山18"和云南野生香菇"11-1"为亲本,通过单孢杂交获得84个杂交菌株。用ISSR分子标记对杂交菌株进行真假鉴定,证明有45个菌株是真正的杂合子。本研究也表明了用ISSR标记可以对香菇杂合子的真实性进行早期鉴定。Lentinula edodes cultivar variety Dashan 18 and yunnan wild strain 11-1 were parental strains, and 84 hybrid strains were acquired by monospore cross breeding. ISSR molecular marker was used to identify true and false of hybrid strains, confirming 45 strains to be true heterozygotes, indicating ISSR molecular marker could be used to early identify authenticity of Lentinula edocles hybrid strains.

关 键 词：香菇 香菇菌株单孢杂交 香菇菌株ISSR鉴定 

分 类 号：S646.12[农业科学—蔬菜学]