一株丙烯酰胺高效降解菌的筛选及其降解性能  

Screening and degradability of an acrylamide degradating bacterium

作　　者：郑国林[1] 周晓见[1,2] 董昆明[1,2] 缪莉[1,2] 靳翠丽[1,2] 姜薇[1,2] 

机构地区：[1]扬州大学环境科学与工程学院,扬州225127 [2]扬州大学海洋科学与技术研究所,扬州225127

出　　处：《环境工程学报》2013年第1期360-364,共5页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(41076097;41006097;41106113);教育部科学技术研究重点项目(211065);江苏省自然科学基金资助项目(BK2010322);江苏省属高校自然科学研究项目(10KJB170013;11KJB170011);江苏省环境材料与环境工程重点实验室开放课题(K090027;K090025;K090026;K090028);扬州大学"新世纪"人才工程支持项目

摘　　要：从污水处理厂的活性污泥中,分离、筛选出1株高效降解丙烯酰胺的菌株A18,经16S rDNA序列分析鉴定该菌株属于Delftia tsuruhatensis,它可以降解苯胺。以丙烯酰胺为惟一碳源的无机盐培养基中,以菌株细胞的增长和丙烯酰胺的降解为依据,通过实验得出A18菌株的最适生长条件:温度为30℃,pH为7.0。在最适生长条件下,当丙烯酰胺的初始浓度约为1 000 mg/L时,菌株A18对丙烯酰胺的48 h降解率达到100%。From an activated sludge in the secondary sedimentation tank of wastewater treatment plant, a bacterial strain with high efficiency of degrading acrylamide was found, and was named as A18 in this study. Based on the result of 16S rDAN sequences, this strain was identified as Delfiia tsuruhatensis, which is reported as a degradation strain for aniline. Using acrylamide as sole source of carbon, the degradation ability of A18 was tested. By the comparison between growth conditions and acrylamide degradation, the optimum degradation conditions were determined, which were temperature of 30℃ and pH of 7.0. Moreover, under the optimum conditions, the acrylamide with initial concentration of 1 000 mg/L could be completely degraded by the strain A18 within 48 h in the inorganic medium.

关 键 词：丙烯酰胺 活性污泥 降解菌 

分 类 号：X172[环境科学与工程—环境科学]