一株分离自中国鹅源黄病毒的分子鉴定  

Molecular identification of a strain of flavivirus isolated from Chinese geese

作　　者：陈玉环[1,2] 葛铭 刘红玉[1,2] 张云 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]中国农业学院哈尔滨兽医研究所生物技术国家重点实验室,哈尔滨150001

出　　处：《黑龙江畜牧兽医》2013年第1期15-18,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31072132);现代农业水禽产业技术体系岗位科学家专项(CARS-43-10)

摘　　要：为了分析2011年分离到的1株(ZZ株)引起产蛋量下降的鹅源黄病毒的全基因组序列及病原的分子特性,试验采用PCR技术对其全基因组进行PCR扩增、克隆和测序,之后对多聚蛋白的剪切位点、潜在的糖基化位点、半胱氨酸的分布及3'UTR的二级结构进行分析。结果表明:该病毒由10986个核苷酸组成,并具有典型的黄病毒基因组结构;开放阅读框(ORF)编码3 425个氨基酸(95～10 372 nt);与其他黄病毒比较,该病毒的5'UTR(94 nt)大小居中,而3'UTR(614 nt)相对较长;对多聚蛋白及3'UTR的系统进化树分析显示,ZZ分离株与恩塔亚病毒(Ntaya virus)的巴格扎病毒(Bagazavirus)亲缘关系最近。To analyze the genome sequence and molecular characterization of the pathogen for the goose-origin flavivirus isolated in China in 2011, which could cause a drop in egg production. The complete genome of the virus was amplified by PCR, cloned into T vector and se-quenced. The polyprotein cleavage sites, potential glycosylation sites, distribution of cysteine residues, and 3 ＇UTR secondary structure were an-alyzed. The results showed that the virus genome consisted of 10 986 nucleotides and had a typical flavivirus genome structure. The open read- ing frame （ORF） encoded 3 425 amino acids （95 - 10 372 nt）. The 94 - nucleotide 5＇UTR was of intermediate size and the 614 - nucleotide 3＇UTR was quite long compared to other flaviviruses. Phylogenetic analysis of the 3＇UTR sequences indicated that the ZZ isolate was closely re-lated to Bagaza viruse from Ntaya virus group.

关 键 词：鹅源黄病毒 分子鉴定 进化分析 

分 类 号：S852.659.6[农业科学—基础兽医学]