茶多酚对CD8＋T细胞杀伤白癜风患者黑素细胞的保护作用研究  

Protective effects of tea polyphenols against the destruction of melanocytes by CD8＋ T cells from vitiligo patients

作　　者：欧阳杰[1] 吴纪龙[1] 周妙妮[1] 付丽芳[1] 许爱娥[1] 

机构地区：[1]杭州市第三人民医院皮肤科,310009

出　　处：《中华皮肤科杂志》2013年第1期20-23,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81071294）;浙江省自然科学基金重点项目（Y2111310,Z2100973）

摘　　要：目的探讨茶多酚对CD8＋T细胞杀伤白癜风患者黑素细胞的保护作用。方法取白癜风患者白斑边缘处皮片组织，培养CD8＋淋巴细胞，细胞纯度用流式细胞仪进行鉴定，通过和黑素细胞共培养验证其针对黑素细胞的杀伤作用。将不同浓度茶多酚加入CD8＋T细胞和黑素细胞共培养的体系，用流式细胞仪检测黑素细胞的凋亡。结果取白癜风皮损边缘表皮组织中成功培养CD8＋T淋巴细胞，纯度达90％以上，高表达活化抗原CD137和CD69。CD8＋T细胞和黑素细胞共培养后，可明显诱导黑素细胞凋亡。200μg／ml和400μg／ml浓度茶多酚处理均可以降低黑素细胞的凋亡率。结论白癜风皮损边缘组织来源的CD8＋T细胞对白癜风患者黑素细胞有明显杀伤作用。茶多酚对抗CD8＋T细胞杀伤黑素细胞，从而对黑素细胞有一定的保护作用。Objective To evaluate the protective effects of tea polyphenols against the destruction of melanoeytes by CD8＋ T cells from vitiligo patients. Methods Skin tissue was resected from the margin of vitiligo lesions followed by the isolation and culture of CD8＋ T lymphocytes, and from the normal skin of vitiligo patients followed by the isolation and culture of melanoeytes. Flow cytometry was carried out to evaluate the purity of CD8＋ T cells. The melanocytes were cocuhured with the CD8＋ T cells at different ratios followed by the evaluation of killing effect of CD8＋ T cells. Various concentrations （200 and 400 Ixg/ml） of tea polyphenols were added into the co-culture system of CD8＋ T cells and melanocytes at a ratio of 5 ： 1 followed by an additional culture of 48 hours. Then, flow eytometry was performed to detect the apoptosis in melanocytes in the cocuhure system. Results CD8＋ T lymphoeytes were successfully obtained from the marginal area of vitiligo lesions with a purity of more than 90%, which highly expressed the antigens CD137 and CD69. The coculture with CD8＋ T cells markedly accelerated the apoptosis in melanocytes, while the accelerative effect was inhibited by tea polyphenols of 200 and 400 I-＋g/ml. Conclusions The CD8＋ T cells infiltrating the edge of vitiligo lesions display a potential destructive effect on autologous melanocytes from vitiligo patients, and tea polyphenols have a protective effect against the destruction of melanocytes by CD8＋ T cells.

关 键 词：白癜风 T淋巴细胞 细胞毒性 黑素细胞 茶多酚 

分 类 号：R758[医药卫生—皮肤病学与性病学]