一个骨髓移植方法建立的小鼠IgA肾病模型  

Establishment of murine IgA nephropathy model by bone marrow transplantation

作　　者：佐楠[1] 姚丽[1] 王力宁[1] 李子龙[1] 范秋灵[1] 冯江敏[1] 马健飞[1] 

机构地区：[1]中国医科大学附属第一医院肾内科,辽宁沈阳110001

出　　处：《中国现代医学杂志》2012年第29期40-44,共5页China Journal of Modern Medicine

摘　　要：目的通过骨髓移植建立IgA肾病动物模型,观察其疾病发展变化规律。方法骨髓移植后的第6、12和24周处死小鼠,留取血尿及肾脏标本。应用PAS染色、免疫荧光法、Western blotting法、免疫组织化学法及ELISA法评价肾组织损伤程度。结果受体鼠在第6周出现系膜IgA的沉积和血清IgA水平的升高,伴有轻度的系膜扩张和微量蛋白尿;第12周和24周时系膜IgA的沉积明显增加,血清IgA和尿蛋白水平进一步升高伴明显的系膜基质扩张和肾小球FN的沉积。24周与12周相比,肾损伤无明显加重。结论以IgA肾病早期发病ddY鼠为供体行骨髓移植可以成功地在C57BL/6小鼠上重建IgA肾病,造模后12周为最佳观察周期。【Objective】 To establish a murine IgA nephropathy（IgAN） model by bone marrow transplantation（BMT） and find the characteristics of this model.【Methods】 The recipients were sacrificed at 6,12 and 24 weeks after BMT and their serum,urine and kidneys were collected.Injury of renal tissue was evaluated by PAS staining,immunofluorescence,Western blotting,immunohistochemistry and ELISA.【Results】 The recipients showed mild mesangial IgA deposition,expansion of mesangial matrix and elevation of serum IgA,accompany with microproteinuria at 6 weeks after BMT.Mesangial IgA deposition,expansion of mesangial matrix,elevation of serum IgA and proteinuria turned more remarkable at 12 and 24 weeks,accompany with significant glomerular fibronectin deposition.But there was no significant difference of renal damage between 12 and 24 weeks.【Conclusion】 BMT can reconstitute IgAN in C57BL/6 mice with IgAN early-onset ddY mice as donors and 12 weeks was the appropriate period for observation.

关 键 词：IGA肾病 骨髓移植 动物模型 

分 类 号：R-332[医药卫生] R692