溶血磷脂酸对在体海马星形胶质细胞增殖的影响  

Study of lysophosphatidic acid induces hippocampus astrocyte proliferation in vivo

作　　者：朱扬[1] 张兆辉[1] 曾伟 高岩[1] 许泽武[1] 徐金梅[1] 

机构地区：[1]武汉大学人民医院神经内科 [2]武汉市第五医院神经内科,湖北武汉430060

出　　处：《中国现代医学杂志》2012年第29期53-56,共4页China Journal of Modern Medicine

摘　　要：目的探讨溶血磷脂酸(LPA)对在体海马星形胶质细胞(AST)的影响。方法在立体定向仪下向大鼠海马CA1区注射50μm LPA,在不同时间点(10 min、30 min、60 min、1 d、3 d、5 d、7 d、14 d、21 d),采用免疫荧光标记法检测注射部位胶原纤维酸性蛋白(GFAP)含量并使用激光共聚焦扫描查看GFAP的分布情况。结果LPA注射后,GFAP阳性细胞数显著增加,与PBS对照组比较差异有统计学意义(P<0.05),并随时间延长,GFAP荧光强度逐渐增加,呈时间依赖性,至LPA注射后3周仍可见增加。结论 LPA可以促进大鼠海马AST增殖,时间至少可以持续3周。【Objective】 To investigate the effect of lysophosphatidic acid（LPA） on hippocampus astrocyte（AST） in vivo.【Methods】 The rat was positioned on a stereotactic frame and PBS（PH=7.4） or 50 μM LPA was injected into the rat hippocampal CA1 area by a needle under stereotactic guidance,at different time points（10 min,30 min,60 min,1 d,3 d,5 d,7 d,14 d,21 d）.Then using immunofluorescence to detect the fibrillary acidic protein（GFAP） content of the injection site and laser confocal scanning to view the distribution of GFAP.【Results】 Compared with the control group,GFAP-positive cells was significantly increased after injecting LPA,there was significant difference（P 0.05）.As times,the fluorescence intensity of GFAP increased gradually,it＇s time-dependent manner,and the proliferation still visibled 3 weeks later.【Conclusion】 LPA stimulated the of hippocampal AST,the proliferation preserved at least 3 weeks.

关 键 词：溶血磷脂酸 星形胶质细胞增殖 激光共聚焦扫描 

分 类 号：R743[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]