香椿子正丁醇提取物对脑缺血再灌注小鼠的神经保护作用及其机制研究  

Neuroprotective effect of n-butanol extract of Chinese toon seeds on mouse with injury of ischemia-reperfusion and its mechanism

作　　者：马迪[1] 陈超[1] 吴薇[1] 游艳[1] 潘宜云[1] 盛梦婷[1] 朱峰[1] 何治[1] 

机构地区：[1]三峡大学医学院、三峡大学国家中医药管理局中药药理三级实验室,湖北宜昌443002

出　　处：《解放军医学杂志》2013年第1期15-18,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金青年基金(81100873);湖北省卫生厅青年科技人才项目(QJX2010-27);三峡大学博士科研启动基金(KJ2008B053)~~

摘　　要：目的探讨香椿子正丁醇提取物(n-BuE)对脑缺血再灌注小鼠的神经保护作用及其机制。方法双侧颈总动脉反复缺血/再灌注合并硝普钠注射降压方法建立脑缺血再灌注小鼠模型。42只小鼠随机分为6组:假手术组、缺血再灌注组、溶媒组、n-BuE[28mg/(kg.d),42mg/(kg.d)]治疗组、阳性对照组[给予14mg/(kg.d)阿司匹林],每组7只。手术后断头取脑,干湿重法测脑组织含水量,伊文思蓝含量测定法观察血脑屏障通透性。分离皮层和海马组织,分光光度法测定丙二醛(MDA)、一氧化氮(NO)含量和过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力变化。结果与假手术组比较,缺血再灌注组脑组织含水量、伊文思蓝含量,海马组织MDA和NO含量、SOD和GSH-Px活力均明显增加(P<0.05或P<0.01)。而与缺血再灌注组比较,n-BuE[28mg/(kg.d),42mg/(kg.d)]治疗组的脑组织含水量、伊文思蓝含量,海马组织MDA和NO含量、SOD和GSH-Px活力均明显减少(P<0.05或P<0.01)。结论 n-BuE可通过其抗氧化应激效应对脑缺血再灌注小鼠发挥神经保护作用。Objective To investigate the neuroprotective effect and its mechanism of n-butanol extract（n-BuE） of Chinese toon seeds on mouse with ischemia-reperfusion（I-R） injury of the brain in vivo.Methods The brain I-R model was established in 42 mice by ligation of bilateral common carotid arteries for 2 times combined with injection of sodium nitroprusside.Animals were randomly divided into 6 groups（7 each）： sham-operated group,I-R model group,vehicle control group,two treatment groups [treated with n-BuE of Chinese toon seeds 28mg/（kg.d） or 42mg/（kg.d）,respectirely,for 7 days],and positive control group [treated with aspirin 14mg/（kg.d）].The animals were sacrificed by decapitation after the experimental procedure,and the water content of brain tissue was determined by weighing.The permeability of the blood brain barrier was evaluated by measurement of Evans blue（EB） content with spectrophotometer.The contents of malondialdehyde（MDA） and nitrogen mono-oxide（NO）,and the activities of superoxide dismutase（SOD） and glutathione peroxidase（GPX） in brain tissue were determined with spectrophotometer.Results The water content and EB content of brain tissue,and the contents of MDA and NO,and the activities of SOD and GPX in hippocampal tissue increased significantly in I-R model group than in sham-operation group（P〈0.05 or P〈0.01）,while they decreased obviously in the two treatment groups when compared with those in I-R model group（P〈0.05 or P〈0.01）.Conclusion n-BuE of Chinese toon seeds may play a neuroprotective effect on the brain tissue I-R injured mice due to its anti-oxidative stress effect.

关 键 词：香椿子 脑缺血 再灌注损伤 

分 类 号：R743[医药卫生—神经病学与精神病学]