炎症细胞因子诱导Tca8113细胞表达PD-L1的作用研究  

Inflammatory Factors Promote the Expression of PD-L1 in Tca8113

作　　者：卢礼兵[1] 陈娇[1] 冯云[1] 周陈晨[1] 张平[1] 

机构地区：[1]四川大学口腔疾病研究国家重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2013年第1期42-45,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.30972764)资助

摘　　要：目的通过研究炎症细胞因子对人舌鳞状细胞癌细胞Tca8113中程序性死亡因子配体-1(programmed death 1ligand-1,PD-L1)的表达的影响,探讨肿瘤炎性微环境在肿瘤细胞免疫逃逸中的作用和机制。方法用流式细胞分析术(FCM)检测Tca8113在各种炎症细胞因子〔白介素(IL)-1β、IL-2、IL-6、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)以及其他诱导条件〔牙龈卟啉单胞菌代谢产物(Pg-sup)及活化外周血单个核细胞的代谢产物(PHA-sup)〕处理下PD-L1的表达变化。结果 PHA-sup、Pg-sup液,IL-1β、IL-6、TNF-α、IFN-γ等炎症相关细胞因子均能诱导Tca8113中PD-L1的表达,与未做处理的Tca8113(对照组)比较差异有统计学意义(P<0.05),但IL-2诱导后PD-L1的表达与对照组的差异无统计学意义(P>0.05)。与单个炎症细胞因子诱导比较,炎症细胞因子组合诱导的PD-L1表达均有增高(P<0.05),炎症细胞因子在诱导PD-L1表达中相互之间存在协同效应,其中,IL-1β+IFN-γ、TNF-α+IFN-γ以及L-1β+IL-6+IFN-γ+TNF-α这3组的表达量高于其他各组(P<0.05)。结论肿瘤微环境炎症细胞因子促进肿瘤细胞表达PD-L1,在肿瘤免疫逃逸中发挥重要作用。Objective To investigate the mechanism of immunologic escape in the tumor microenvironment, study the expression of programmed death 1 ligand-1 （PD-L1） in Tca8113 with treatment of inflammatory factors. Methods The expression of PD-L1 treated with inflammatory factors （IL-1β, IL-2, IL-6, TNF-α, IFN-γ） was detected by flow cytometry （FCM）. Results The expression of PD-L1 in Tca8113 was up-regulated conspicuously with treatment of inflammatory factors （P〈0.05）, including.. IL-1β, IL-6, TNF-α, IFN-γ. And the factors played the role in synergistic effects, most significantly in the groups of IL-1β＋IFN-γ, TNF-α IFN-γ and IL-1β-IL-6＋ IFN-γ＋TNF-α. But the influence of IL-2 on the expression of PD-L1 was not significant （P〉0.05）. Conclusion With the up-regulated expression of PD-L1, the tumor would be escaped more easily from the immunoreactions, while there were an abundance of inflammatory factors in the tumor microenvironment.

关 键 词：炎症细胞因子 程序性死亡因子配体-1 TCA8113 

分 类 号：R739.86[医药卫生—肿瘤]