氟尿嘧啶对人结肠癌SW480细胞ABCG2表达的影响  

Effect of 5-fluorouracil on the expression of ATP-binding cassette superfamily G member 2 in human colon cancer cell SW480

作　　者：瞿金妙[1] 尤捷[1] 刘海光[1] 黄奇迪[1] 郭贵龙[1] 

机构地区：[1]温州医学院附属第一医院肿瘤外科,325003

出　　处：《中华胃肠外科杂志》2013年第1期89-92,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的观察氟尿嘧啶（5-FU）对人结肠癌SW480细胞ABCG2表达的影响。方法用不同药物浓度的5-FU处理SW480细胞，用CCK8法检测5-FU在SW480中的IC50，流式细胞仪检测SW480细胞ABCG2的阳性表达率．RT—PCR检测ABCG2的mRNA在SW480细胞中的表达差异。结果5-FU对SW480细胞的IC50随着药物浓度的增加而升高（P〈0．05）。流式细胞仪检测发现，正常SW480细胞（A组）中ABCG2阳性表达率为（6．26±0．86）％；在药物处理48h后即刻检测时（B组）的阳性表达率下降至（3．43±1．18）％（P〈0．05）；在药物处理48h后的第2代细胞检测时（c组）则升高至（12．91±3．42）％（P〈0.05）。3组ABCG2mRNA表达趋势与流式细胞仪检测结果的趋势一致。结论不同浓度的5-FU可以影响人结肠癌SW480细胞ABCG2的表达。Objective To investigate the effect of 5-fluorouracil （5-FU） on the expression of ATP-binding cassette superfamily G member 2（ABCG2）in human colon cancer cell SW480. Methods SW480 cells were treated with various concentrations of 5-FU. CCK8 assay was utilized to detect the 5-FU IC50 to SW480 cells. Positive expression of ABCG2 was detected by flow cytometry, and mRNA expression of ABCG2 was detected by real time polymerase chain reaction （RT-PCR）. Results The 5-FU IC50 to SW480 cells increased as the drug concentration increased（P〈0.05）. Flow cytometry revealed that positive expression rate of ABCG2 in normal SW480 cells （group A） was （6.26±0.86）%. Immediately after treatment with 5-FU for 48 hours, the positive expression rate of ABCG2 （group B） was （3.43±1.18）%（P〈0.05）. In the second passage of cells after treatment with 5-FU for 48 hours, the positive expression rate of ABCG2 （group C） was （12.91±3.42）%（P〈0.05）. The mRNA expression of ABCG2 detected by RT-PCR was in accordance with the results from flow cytometry. Conclusion Expression of ABCG2 in SW480 cells can be affected by various concentrations of 5-FU.

关 键 词：结肠肿瘤 氟尿嘧啶 SW480细胞 ABCG2 

分 类 号：R735.35[医药卫生—肿瘤]