遗传性和散发性胃癌上皮型钙黏附素的表达和启动子甲基化状态的关系  

E-cadherin expression and CDH1 promoter methylation in sporadic and hereditary gastric cancer

作　　者：李晓军[1] 赵阳[2] 任宏[1] 

机构地区：[1]西安交通大学医学院第一附属医院胸外二科,陕西西安710061 [2]西安交通大学医学院第二附属医院肿瘤外科,陕西西安710004

出　　处：《南方医科大学学报》2013年第1期125-127,共3页Journal of Southern Medical University

基　　金：陕西省攻关项目(2010R01-130)

摘　　要：目的检测上皮型钙黏附素的表达和其基因(CDH1)的启动子甲基化情况,探讨上皮型钙黏附素在家族遗传性胃癌和散发性胃癌发生中的作用。方法收集符合诊断标准的家族性遗传性胃癌19例、不伴胃癌家族史散发性胃癌19例,正常胃粘膜组织对照组19例。采用免疫组织化学方法检测上皮型钙黏附素表达情况,采用甲基化特异的PCR检测基因启动子甲基化情况。结果上皮型钙黏附素在遗传性和散发性胃癌组的表达均有明显下调,与正常组织的差异具有统计学意义(P<0.001)。遗传性胃癌和散发性胃癌的上皮型钙黏附素均降低,但两组差异没有统计学意义(P=0.84)。正常组织中未检测到启动子甲基化,遗传性胃癌中3例检测到甲基化,散发性胃癌组中检测到10例启动子甲基化,散发性胃癌组的甲基化率高于遗传性胃癌组(P<0.01)。结论CDH1基因启动子区甲基化可能是导致散发性胃癌上皮型钙黏附素表达下调的重要原因,但可能不是遗传性胃癌的上皮型钙黏附素表达下调的主要作用机制。Objective To evaluate the role of E-cadherin （E-cad） and CDHI gene encoding E-cad in the occurrence of sporadic or hereditary gastric cancer. Methods Nineteen normal gastric mucosal issue specimens, 19 specimens of hereditary gastric cancer （diagnosed according to ICG-HGC criteria）, and 19 specimens of sporadic gastric cancer examined for E-cad expression and CDH1 promoter methylation using immunohistochemistry and methylation-specific PCR （MSP）. Results The protein expression of E-cad were significantly reduced in both of the cancer tissues （P〈0.001） compared with that in the normal gastric mucosal tissues, and showed no significant difference between the two cancers （P=0.84）. CDH1 promoter hypermethylation was found in 10 out of the 19 hereditary gastric cancer tissues, a rate significantly higher than that in sporadic gastric cancer tissues （3/19, P〈0.01）. Conclusion CDH1 promoter hypermethylation is probably an important factor contributing to reduced E-cad expression in sporadic gastric cancer but not in hereditary gastric cancer.

关 键 词：胃癌 上皮型钙黏附素 CDH1基因 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]