IGF1和IGF1R及AKT与卵巢癌顺铂耐药相关性研究  

Relationshiop between IGF1,IGF1R and Akt and cisplatin resistance of ovarian cancer

作　　者：贾美群[1] 陈曾燕[1] 吴霞[1] 刘继斌[1] 吴银芳[1] 

机构地区：[1]南通市肿瘤医院妇瘤科,江苏南通226361

出　　处：《中华肿瘤防治杂志》2012年第22期1734-1737,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：南通市社会发展科技项目(2010016)

摘　　要：目的:检测胰岛素生长因子(IGF)信号通路关键蛋白IGF-1、IGF-1R及Akt在顺铂耐药卵巢癌患者血清中的表达,及其与多药耐药蛋白MRP2的相关性。方法:利用ATP-TCA法对40例卵巢癌标本进行药敏试验,其中16例对顺铂耐药,24例对顺铂敏感。ELISA法分别检测顺铂耐药和敏感组患者血清中IGF-1、IGF-1R及Akt和MRP2的表达,进行相关性分析。结果:IGF-1、IGF-1R及Akt在卵巢癌顺铂耐药组的表达显著高于敏感组,P值均为0.000 1;MRP2的表达卵巢癌顺铂耐药组高于敏感组,P=0.035;IGF-1和IGF-1R有显著相关性,r=0.755,P=0.000 1;IGF-1、IGF-1R和Akt有显著相关性,r值分别为0.812和0.643,P值均为0.000 1;MRP2和IGF-1有相关性,r=0.493,P=0.018;IGF-1R与MRP2无相关性,r=0.231,P=0.173;Akt和MRP2无相关性,r=0.274,P=0.106。结论:IGF信号通路关键蛋白IGF-1、IGF-1R及Akt可能参与卵巢癌顺铂耐药,IGF-1与多药耐药蛋白有一定的相关性,IGF-1可能作为卵巢癌靶向治疗的新靶点。OBJECTIVE：To detect the levels of insulin-like growth factor signaling pathway key protein of insulinlike growth factor I（IGF-1）,insuliwlike growth factor 1 receptor （IGF-1R） and Akt in cisplatin-resistance ovarian cancer,and explore the correlations between expressions of IGF4, IGF-1R, Akt and MRP2. METHODS： Drug resistance test was detected by ATP-TCA in forty ovarian cancer tissue, sixteen of cisplatin resistance, twenty-four of cisplatin sensitive. The expressions of IGF-1 ,IGF-1R,Akt,MRP2 were detected by ELISA in ovarian patient blood,and the correlations between IGF-I,IGF-1R,Akt and MRP2 were studied. RESULTS： The expressions of IGF-1 ,IGF-1R,Akt in cisplatin resistance group were significantly higher than that in cisplatin sensitive group （P=0. 000 1）. The expressions of MRP2 in cisplatin resistance group were significantly higher than that in cisplatin sensitive group （P = 0. 035）. There was positive correlation between IGF-1 and IGF-1R （r= 0. 755, P = 0. 000 1）, and positive correlation between IGF-1, IGF-1R and Akt （r were 0. 812 and 0. 643, and all P were 0. 000 1）, and positive correlation between IGF-1 and MRP2 （r= 0. 493, P= 0. 018）,and no correlation between MRP2 and IGF-1R （r= 0. 231,P=0. 173）,no correlation between MRP2 and Akt （r=0. 274,P=0. 106）. CONCLUSIONS： Cisplatin-resistance in ovarian caneer is strongly correlated with the expressions of IGF-1, IGF-1R and Akt. There is positive correlation between IGF1 and MRP2. IGF-1 is an attractive target for ovarian cancer treatment.

关 键 词：卵巢肿瘤 胰岛素生长因子1 胰岛素生长因子1受体 蛋白激酶B 多药耐药蛋白 顺铂 

分 类 号：R737.31[医药卫生—肿瘤]