环介导等温扩增技术及其在病原微生物检测中的应用  

Loop-mediated isothermal amplification technology and its application in pathogenic microorganism detection

作　　者：肖维威[1] 周琳华[1] 郑文岭[1] 马文丽[1] 

机构地区：[1]南方医科大学基因工程研究所,广东广州510515

出　　处：《基础医学与临床》2013年第1期19-23,共5页Basic and Clinical Medicine

摘　　要：环介导等温扩增(LAMP)技术是近年发展起来的一项新的快速核酸恒温扩增技术,具有高效、快速、特异、易检测、易操作等特点,自面世以来被科学家认为是能替代常规PCR的一项扩增技术,非常适用于现场检测和基层检测。本文就LAMP技术的原理、特点、进展及其在病原微生物检测中的应用进行了简要的概述。Loop-mediated isothermal amplification（LAMP） technology is a novel nucleic acid isothermal amplification technology developed in recent years.Because of its high effiency,rapidity,specificity,and simplicity,scientists believe it will be a routine amplification technology replacing the conventional PCR method.This paper briefly reviews the principle of the technology,techinical characteristics,progress,and its application in pathogenic microorganism detection.

关 键 词：环介导等温扩增 检测 病原体 

分 类 号：R446.5[医药卫生—诊断学]