人骨髓间充质细胞脑内移植治疗食蟹猴脑缺血  

Human mesenchymal stem cell transplantation promotes functional recovery of macaca fascicularis with cerebral ischemia

作　　者：朱华[1] 冯铭[2] 卢珊[3] 李秦[4] 刘颖[1] 安沂华[5] 赵春华[3] 王任直[2] 秦川[1] 

机构地区：[1]中国医学科学院北京协和医学院比较医学中心卫生部比较医学重点实验室,北京100021 [2]中国医学科学院北京协和医学院北京协和医院,北京100730 [3]中国医学科学院北京协和医学院基础医学研究所,北京100005 [4]中国医学科学院北京协和医学院实验动物研究所Motac合作实验室,北京100021 [5]中国医学科学院北京协和医学院北京神经外科研究所,北京100039

出　　处：《基础医学与临床》2013年第1期33-38,共6页Basic and Clinical Medicine

基　　金：国家高技术研究发展计划(2006AA02A115)

摘　　要：目的评价人骨髓间充质干细胞脑内移植对食蟹猴脑缺血模型的治疗作用。方法健康成年食蟹猴18只,光化学法造模4周后,用脑立体定位法在缺血灶周围植入人骨髓间充质干细胞5×106和1×106,对照组等体积0.9%氯化钠注射液。利用影像学(MRI、PET)、行为学(神经功能评分、精准上肢测试系统)和组织病理学对干细胞移植效果进行评价。结果神经功能评分和精准上肢运动测试分别在在干细胞植入2周和3 d后显著降低(P<0.05)。PET结果显示干细胞移植后4、6周高、低剂量组缺血灶周围皮质、基底节核团的SUV%值比对照组显著升高(P<0.05)。MRI显示剂量组缺血灶的修复速度大于对照组。病理检查可见低剂量组坏死灶面积小于对照组(P<0.05),出血灶周围有大量新生血管生成。结论在缺血灶周围植入hBMSC可促进食蟹猴损伤神经组织的恢复,为进一步研究干细胞治疗神经系统疾病的临床应用提供了参考。Objective By using human bone marrow-derived mesenchymal stem cell（hBMSC） transplantation to treat experimentally induced cerebral ischemia in Macaca fascicularis to examine the effects of hBMSC transplantation on the recovery of neurological function.Methods In 18 Macaca fasciculari,cerebral ischemia was induced by photochemical occlusion to generate the stoke model.The animals were randomly divided into high-dose,low-dose,and control groups.After 4 weeks,250 μL of hBMSC at 5×106,1×106,or saline were injected respectively at the edge of the hematoma.High field MR scanning and positron-emission tomography（PET） scans were performed every 2 weeks before and after the hBMSC transplant action.Neurological functioning and upper limb motor test were used to evaluate on 24hrs,3 dand 1,2,3,4,5,6,7,8,9,10,12 weeks after the surgery.Results After hBMSC transplantation,neural function scores and upper limb motor test improved significantly in the high-and low-dose groups compared with the control group.4 and 6 weeks after the transplantation,SUV% of the surrounding area of the lesion had significant difference between dose group and control group（P0.05）.At week 4 and 12,pathological examination showed a large amount of angiogenesis around the lesion.The area of the lesion of low-dose group decreased significant as compared to the control group（P0.05）.Conclusions Intracerebral transplantation of human mesenchymal stem cells can improve functional recovery of Macaca fascicularis and can be used to restore neurological deficits in experimentally induced ischemia stroke.

关 键 词：间充质干细胞 脑缺血 移植 食蟹猴 

分 类 号：R743.3[医药卫生—神经病学与精神病学]