5-氮-2'-脱氧胞苷逆转膀胱癌细胞hepaCAM基因的表达及对其生长的抑制  

5-aza-2′-deoxycytidine reverses the expression of hepaCAM mRNA and inhibits the growth of T24 and BIU-87 cells

作　　者：刘琪[1] 潘翠翠[1] 徐新[1] 张彦懿[1] 吴小候[2] 罗春丽[1] 

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室重庆市重点实验室,重庆400016 [2]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《基础医学与临床》2013年第1期55-59,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81072086)

摘　　要：目的研究5-氮-2-脱氧胞苷(5-aza-CdR)逆转hepaCAM表达及抑制膀胱癌细胞的生长。方法 MTT法检测细胞增殖;流式细胞术检测细胞凋亡;RT-PCR检测hepaCAM mRNA的表达。结果在T24细胞中,3.0和10.0μmol/L5-aza-CdR药物浓度的抑制率明显高于0.3和1.0μmol/L 5-aza-CdR(P<0.05),在BIU-87细胞中,5.0μmol/L5-aza-CdR药物浓度的抑制率明显高于0.1和0.5μmol/L(P<0.05),并且药物处理细胞72 h的抑制率明显高于24和48 h(P<0.05);5-aza-CdR处理细胞后,T24和BIU-87细胞凋亡数明显增加(P<0.05);并可逆转hepaCAMmRNA表达。结论 5-aza-CdR可使hepaCAM基因重新表达,并通过诱导凋亡而抑制膀胱癌细胞的生长。Objective To investigate the effect of 5-aza-CdR on expression of hepaCAM mRNA and the inhibition of T24 and BIU-87cell lines.Methods The effects of 5-aza-CdR on cell proliferation of T24 and BIU-87 cell lines were measured by MTT;Flow cytometry was used to examine apoptosis of T24 and BIU-87 cells;RT-PCR was used to detect the expression of hepaCAM in T24 and BIU-87 cell lines with or without 5-aza-CdR treatment.ResultsThe inhibition ratios of 3.0 and 10.0 μmol/L 5-aza-CdR were significantly higher than that of 0.3 and 1.0 μmol/L 5-aza-CdR in T24 cell（P0.05）.The inhibition ratio of 5.0 μmol/L 5-aza-CdR was higher than that of 0.1 and 0.5 μmol/L 5-aza-CdR in BIU-87 cell（P0.05）.Cell apoptotic rate increased in cells with 5-aza-CdR treatment（P0.05）.The expression of hepaCAM mRNA was reversed in T24 and BIU-87 cells after treatment.Conclusions 5-aza-CdR can reverse the expression of hepaCAM mRNA and therefore inhibit the cell growth through inducing apoptosis in bladder cancer tissue.

关 键 词：5-AZA-CDR hepaCAM 膀胱癌 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤]