重组人粒细胞集落刺激因子促进兔手术联合化疗后的切口愈合  

Recombinant human granulocyte colony stimulating factor accelerates wound healing after treatment of chemotherapy and surgery

作　　者：徐鲲[1,2] 杨敏[3] 孙静[4] 闫荣[2] 吕宝玉[2] 

机构地区：[1]山东大学护理学院,山东济南250012 [2]山东省肿瘤医院,山东济南250117 [3]山东大学齐鲁医院,山东济南250012 [4]山东中医药大学病理教研室,山东济南250355

出　　处：《基础医学与临床》2013年第1期102-105,共4页Basic and Clinical Medicine

摘　　要：目的评价重组人粒细胞集落刺激因子(rhG-CSF)对手术同时使用化疗的兔切口感染程度及血管内皮生长因子(VEGF)和肿瘤坏死因子(TNF-α)的影响。方法新西兰大白兔24只,随机分为3组,空白组单纯进行手术,常规换药;对照组和治疗组均于手术前当日给予表阿霉素静脉输注,常规换药,治疗组换药时涂抹重组人粒细胞集落刺激因子。于手术后第7天观察切口愈合等级,进行病理检查,通过免疫组化检测各组VEGF表达水平,酶联免疫吸附法检测TNF-α水平。结果使用重组人集落刺激因子后切口愈合情况及等级均优于对照组(P<0.05),VEGF表达水平升高,而TNF-α与对照组相比无明显差别。结论重组人粒细胞集落刺激因子可以提高切口部位VEGF表达水平,对手术同时使用化疗的切口有促进愈合作用。Objective To study the effect of rh-GCSF on wound healing after chemotherapy and surgery in rabbits,and the effect of VEGF and TNF-α.Methods New Zealand white rabbits,were randomly divided into 3 groups： normal group（8 cases） was given surgery and medication,control group（8 cases） and treatment group（8 cases） were given infusion of epirubicin on the day before the operation,control group was treated as routine and treatment group was given rh-GCSF.Results The differences were observed including wound healing,VEGF and TNF-α in 7th day after surgery.The VEGF in treatment group was increased and the wound healing was better than the control group,but there was no difference of TNF-α in treatment and control group.Conclusions The rh-GCSF can increase the VEGF expression of incision sites,and facilitate wound healing after treatment with both chemotherapy and surgery.

关 键 词：重组人粒细胞集落刺激因子 伤口愈合 化疗 VEGF TNF-Α 

分 类 号：R730.58[医药卫生—肿瘤]