兰州地区急性呼吸道感染患儿冠状病毒NL63的检测与临床研究  

The detection and clinical feature of HCoV-NL63 in children with acute respiratory tract infection inLanzhou city

作　　者：金玉[1] 张蓉芳[3] 谢志萍[2] 高寒春[2] 闫坤龙[1] 原新慧[3] 宋靖荣[3] 侯云德[2] 段招军[2] 

机构地区：[1]南京医科大学附属南京儿童医院 [2]中国疾病预防控制中心病毒病预防控制所 [3]兰州大学第一医院

出　　处：《中华实验和临床病毒学杂志》2012年第6期409-411,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的探讨兰州地区冠状病毒NL63（HCoV—NL63）在急性呼吸道感染患儿中的流行现状及临床特点。方法收集2006年11月至2009年10月兰州大学第一附属医院急性呼吸道感染（ARTIs）患儿1169例鼻咽分泌物，应用RT—PCR方法检测HCoV-NL63以及其余7种常见呼吸道病毒：鼻病毒（HRV），呼吸道合胞病毒（RSV），偏肺病毒（hMPV），流感病毒（IFVA，IFVB）副流感病毒1．3（HPIVl—3）及PCR方法检测腺病毒（ADV），博卡病毒（HBoV）。结果检测出HCoV—NL63阳性标本35例，检出率2．99％，2007年8、9月，2009年7、8月检测阳性标本阳性率较高，分别为23．53％、17．65％，50％、33．33％。2007年12月至2009年2月未检出HCoV—NL63阳性标本。25（25／35）例混合其他病毒感染，混合感染率为71．43％，最常见的混合感染病毒是HRV。3岁及以下和3岁以上HCoV—NL63感染组感染率差异无统计学意义。HCoV—NL63阳性患儿主要的诊断是支气管肺炎和毛细支气管炎，主要的症状是发热和咳嗽。HCoV-NL63单独感染组和混合感染组除消化道症状外，在其余症状和临床诊断方面，差异均无统计学意义。结论HCoV—NL63是兰州地区呼吸道感染患儿的重要病原，夏季是兰州地区HCoV—NL63感染高峰期，HCoV—NL63的流行存在年度差异。HCoV—NL63感染存在很高的混合感染率，混合感染并不加重HCoV—NL63感染的病情。Objective To investigate the prevalence and clinical characterization of HCoV-NL63 （ NL63 ） in children with acute respiratory tract infections （ARTIs） in Lanzhou with other respiratory viruses. The prevalence of HBoV1 in ALRTI was obviously city,China. Method From November 2006 to October 2009,1169 nasopharyngeal aspirates （NPA） were collected from children under 14 years old with ARTIs. Samples were screened for NL63 using a reverse transcription-polymerase chain reaction （RT-PCR） and sequencing. Demography and clinical information were recorded. Result NL63 was detected in 35 （2. 99% ） of the 1169 children. The peak of the positive rate were in August, september 2007, July, August 2008 （23.53% ,17.65% ,50% ,33.33% separately）. There are no NL63 positive samples was detected in December,2007 to February 2009.25 （25/35,71.43%）were co-infected with other respiratory viruses, and human rhinovirus（HRV） were the most common additional respiratory virus. No significant differences of infective rate of NL63 was found between ~〈 3 years age group and 〉 3 years age group. Bronchiolitis and pneumonia were the most frequent diagnoses in NL63 positive patients and the major symptoms were fever and cough in our study. Between the monoinfection group and the coinfection group of NL63-positive patients, no differences were found in symptoms and clinical diagnoses except symptoms of gastrointestinal~ Conclusion HCoV-NL63 is an important pathogen of acute respiratory tract infection in children in Lanzhou city. The peak of HCoV-NL63 infections was in summer. There were annual differences in the prevalence ofHCoV-NL63. HCoV-NL63 infections existed a high rate of mixed infection,, and mixed infection does not increase the severity of the disease.

关 键 词：呼吸道感染 冠状病毒属 逆转录聚合酶链反应 儿童保健服务 

分 类 号：R72[医药卫生—儿科]