临床体液标本侵袭性真菌感染的早期分子学诊断研究  

Study of the early molecular diagnosis methods in lnvasive fungal infection of clinical humoral specimens

作　　者：冯文莉[1] 王艳青[1] 杨静[1] 奚志琴[1] 王艳[1] 马彦[1] 乔祖莎[1] 

机构地区：[1]山西医科大学第二医院皮肤性病科,太原030001

出　　处：《中国医师杂志》2012年第12期1589-1591,共3页Journal of Chinese Physician

基　　金：山西省基础研究项目资助（2012011045-3;2012011035-3）;山西医科大学科技创新基金（01201013）

摘　　要：目的探讨采用PCR方法检测临床体液标本真菌感染的可行性。方法对60例可疑侵袭性真菌感染者无污染体液标本进行真菌直接镜检及培养，同时应用真菌通用引物ITS4和ITS86对临床体液标本进行PCR检测，分析二者检测结果的差异。结果临床含真菌体液标本直接进行PCR扩增的片段大小均与阳性对照DNA扩增结果一致。且PCR扩增的阳性率与真菌直接镜检、培养方法阳性率相近[38．3％（23／60）V833．3％（20／60），P〉0．05]。结论侵袭性真菌感染采用真菌ITS通用引物进行PCR扩增，能准确、快速地检测出致病真菌，其方法对侵袭性真菌感染的早期诊断与预后具有指导意义。Objective The aim of this study was to investigate the diagnostic impact of polymerase chain reaction （PCR） assays for fungal pathogens in fluid samples, and to evaluate the feasibility of fast PCR diagnostic method for the invasive fungal infection. Methods The sterility body fluid samples from 60 cases hospitalized immunocompromised patients with clinical underlying diseases and suspect of invasive in- fections with fungi between January 2008 and December 2011 were processed for microscopy and cultures. Applying fungal ITS4 and ITS86 universal primers to amplify pathogenic fungi genes from the sterility body fluid with method of rapid PCR. The results were compared between the standard and PCR methods. Results Humoral direct clinical specimens by PCR amplification of DNA fragments with the scan resuhs were similar. Positive rate of PCR test with clinical body fluid samples and the traditional fnngal cultivation was similar. There was no significant statistical difference [38.3% （23/60） vs 33.3% （20/60）, P 〉0.05 ]. Conclusions PCR test is feasibility with clinical fungal diagnosis from directly humoral specimens. To amplify the clinical sterility body fluid samples with ITS fungal universal primers and PCR method might provide an accurate and rapid approach to detect the pathogenic fungi. Its methods on early diagnosis and prog- nosis of invasive fungal infections are of guiding significance.

关 键 词：标本 体液 微生物学 真菌病 诊断 聚合酶链反应 早期诊断 

分 类 号：R519[医药卫生—内科学]