四种细胞移植重建C57BL雌鼠免疫功能的对比  

Four kinds of cells transplantation for reconstructing immune function of C57BL female mice

作　　者：阮光萍[1] 王金祥[1] 姚翔[1] 邓永丽[1] 庞荣清[1] 蔡学敏[1] 王强[1] 马丽花[1] 潘兴华[1] 

机构地区：[1]解放军昆明总医院干细胞与组织器官工程研究中心,云南省昆明市650032

出　　处：《中国组织工程研究》2012年第45期8465-8470,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目资助(31172170);中国博士后基金特别资助(201104748)~~

摘　　要：背景:据报道肌肉来源的细胞能重建造血功能,还需要进一步观察其他系细胞是否也能重建造血功能。目的:对比不同细胞对C57BL雌鼠造血功能恢复的作用。方法:摘眼球取绿色荧光蛋白转基因雄鼠外周血,溶血后制成白细胞,再取脾脏、肝脏制成脾细胞和肝细胞悬液,同时制备骨髓细胞。将C57BL雌鼠半致死量射线照射后,回输4种细胞,并在移植后18,39,53d检测外周血绿色荧光蛋白阳性细胞。同时标记CD4-PE和CD8-PE,并在移植后100d杀鼠取骨髓检测Y染色体阳性率。结果与结论:移植后18,39,53d均在C57BL雌鼠外周血检测到绿色荧光蛋白阳性细胞;C57BL雌鼠外周血绿色荧光蛋白阳性细胞百分比:回输脾细胞和骨髓细胞>回输肝细胞>回输外周血白细胞。同时在回输脾细胞和骨髓细胞的C57BL雌鼠外周血中检测到绿色荧光蛋白和CD4双阳性细胞、绿色荧光蛋白和CD8双阳性细胞,而回输肝细胞和外周血白细胞的C57BL雌鼠外周血中几乎未检测到双阳性细胞。移植后100d检测到的Y染色体阳性率与外周血中绿色荧光蛋白阳性率呈正相关。结果说明绿色荧光蛋白转基因雄鼠的外周血白细胞、脾细胞、肝细胞和骨髓细胞均有重建造血功能的作用,其中脾细胞与骨髓细胞作用相似,作用大于肝细胞,肝细胞作用大于白细胞。BACKGROUND： It is reported that muscle-derived cells can rebuild the hematopoietic function, whether the other cells can rebuild the hematopoietic function also needs further observation. OBJECTIVE： To compare the effect of different cells on the recovery of hematopoietic function of the C57BL female mice. METHODS： We removed the eyeballs of green fluorescent protein transgenic male mice in order to collect the peripheral blood. Then the bloods were hemolyzed to prepare white blood cells. And then, the spleen and the liver were obtained to prepare the spleen cells and liver cells suspension. The bone marrow cells were prepared. The C57BLfemale mice were irradiated by half-lethal dose and transfused with four kinds of cells. At 18, 39 and 53 days after transplantation, green fluorescence protein-positive cells in peripheral blood were detected. At the same time, the bloods were marked with CD4-PE and CD8-PE. At 100 days after transplantation, mice were sacrificed and their bone marrows were collected to detect Y chromosome-positive rate. RESULTS AND CONCLUSION： At 18, 39 and 53 days after transplantation, green fluorescence protein-positive cells were detected in peripheral blood of C57BL female mice; the percentage of green fluorescence protein-positive cells in the peripheral blood of C57BL female mice： transfused with spleen cells and marrow cells 〉 transfused with liver cells 〉 transfused with peripheral white blood cells. At the same time, the CD4 and green fluorescence protein double positive cells and CD8 and green fluorescence protein double positive cells were also detected in the peripheral blood of C57BL female mice after transfused with sDleen cells and marrow cells, while the double positive cells were not detected in the

关 键 词：绿色荧光蛋白 转基因鼠 C57BL雌鼠 移植 细胞移植 骨髓细胞 造血功能 造血重建 荧光原位杂交 肝细胞 白细胞 脾细胞 

分 类 号：R394.2[医药卫生—医学遗传学]