咖啡因对鼠牙胚发育的影响  

Effect of caffeine on rat tooth germ development

作　　者：刘树发[1] 王晓伟[1] 王立峰[1] 霍东英[1] 谷晓明[1] 

机构地区：[1]佳木斯大学附属第二医院颌外一科,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究》2012年第46期8625-8628,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:孕期饮用咖啡会导致孕妇钙吸收障碍,影响胎儿牙胚发育。目的:观察咖啡因对鼠牙胚发育的影响。方法:将Wistar孕鼠随机分为2组,在咖啡因组大鼠受孕7d后的饮水中添加咖啡因,对照组孕鼠饮水中不加咖啡因。孕20d时,两组各处死一半孕鼠取胎鼠,苏木精-伊红染色观察牙胚组织形态;另一半孕鼠连续用药至产仔后20d,测定仔鼠血清中钙离子水平,并取仔鼠第一磨牙进行扫描电镜观察。结果与结论:咖啡因组仔鼠血清中钙离子水平低于对照组(P<0.05)。苏木精-伊红染色可见咖啡因组胎鼠与相同时期对照组胎鼠相比,牙胚组织体积减小,发育迟缓。电镜结果显示咖啡因组孕鼠所产仔鼠的第一磨牙酸蚀后表面空隙比对照组多。提示对孕鼠使用咖啡因可以影响其仔鼠牙胚的发育,导致牙胚发育迟缓,使釉质基质钙化不全,发育后的牙齿不耐酸蚀。BACKGROUND：Coffee drinking during pregnancy can cause calcium malabsorption in pregnant women and affect fetal tooth development.OBJECTIVE：To observe the effect of caffeine on rat tooth germ development.METHODS：The Wistar pregnant rats were randomly divided into two groups.In the caffeine group,caffeine was added into the drinking water at 7 days after pregnancy;and in the control group,no caffeine was added into the drinking water.At 20 days after pregnancy,half of pregnant rats in each group were sacrificed to take the fetal rat,and hematoxylin-eosin staining was performed to observed the morphology of tooth germ;the rest half of the pregnant rats in the two groups were administrated till 20 days after delivery,the calcium level in the serum of rat pups was detected and the first molar of rat pups was observed by scanning electron microscope.RESULTS AND CONCLUSION：The calcium ion content in the serum of rat pups in the caffeine group was lower than that in the control group（P0.05）.Hematoxylin-eosin staining showed that compared with the control group,the volume of the tooth germ tissue of the fetal rats in the same period in the caffeine group was reduced and the development was delayed.Electron microscopy results showed that the surface voids on the first molar of the rat pups in the caffeine group after acid etching was more than those in the control group.Caffeine application in the pregnant rats can inhibit the tooth germ development of rat pups,lead to the insufficiency of enamel matrix calcification,and also result in the intolerance etching of the tooth after development.

关 键 词：咖啡因 牙胚 发育迟缓 影响因素 孕鼠 血清钙离子 酸蚀 大鼠 组织构建 

分 类 号：R318[医药卫生—生物医学工程]