肝细胞癌中NOS和VEGF的表达及其与肿瘤血管形成的关系  

Nitric oxide synthase and vascular endothelial growth factor expression in hepatocellular carcinoma and their relation to angiogenesis

作　　者：王鲁[1] 汤钊猷[1] 孙惠川[1] 叶胜龙[1] 纪元[2] 陆洪芬[3] 施达仁[3] 

机构地区：[1]上海医科大学中山医院肝癌研究所,200032 [2]上海医科大学中山医院病理科,200032 [3]上海医科大学肿瘤医院

出　　处：《中华肿瘤杂志》2000年第4期301-303,共3页Chinese Journal of Oncology

基　　金：上海市医学领先学科基金;美国中华医学基金会基金资助项目 !(93 5 83) ;国家重点基础研究 (恶性肿瘤 )基金资助项目!(G19980 5 1

摘　　要：目的　研究Ⅱ型、Ⅲ型一氧化氮合酶 (iNOS、eNOS)和血管内皮生长因子 (VEGF)在肝细胞癌中的表达及其与肿瘤血管形成的关系。方法　应用免疫组化方法检测 71例肝细胞癌患者手术切除石蜡包埋标本的iNOS、eNOS、VEGF的表达 ,抗CD34单克隆抗体显示血管内皮细胞。根据CD34阳性的血管内皮细胞计数来测定肿瘤微血管密度 (MVD)。结果　iNOS、eNOS分别在 83 .1%、85 .9%的肝癌中阳性表达 ,而正常肝组织不表达。iNOS阳性的肝癌组织 5 9例MVD为 34.3± 1.5 /高倍视野 ,iNOS阴性的组织为 31.2± 2 .8/高倍视野 ,两者差异有显著性 (P <0 .0 1) ;VEGF阳性肝癌组织 6 1例MVD为 38.6± 1.6 /高倍视野 ,VEGF阴性组织为 2 2 .4± 2 .0 /高倍视野 ,两者差异有显著性 (P <0 .0 1)。VEGF、iNOS表达均阳性者 42例 ;VEGF表达阴性、而iNOS表达阳性者 17例。VEGF、eNOS表达均阳性者 43例 ;VEGF表达阴性、而iNOS表达阳性者 18例。表明iNOS、eNOS的表达与VEGF的表达无相关性 (P >0 0 5 )。结论　iNOS、eNOS在肝炎后肝硬化的恶性转化中起作用 ,iNOS。Objective To analyse inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) protein expression in hepatocellular carcinoma (HCC) and their relation to angiogenesis.Methods Tissue sections from 71 HCC patients were examined immunohistochemically for protein expression of iNOS, eNOS, and VEGF. Microvessal density (MVD) was counted by endothelial cells immunostained by anti CD34 antibody.Results Positive iNOS and eNOS immunostaining was detected in 83.1% and 85.9% of HCC respectively. iNOS and eNOS were not detected in normal hepatic tissue. MVD was 34.3±1.5/HP and 38.6±1.6/HP in HCC positive for iNOS and VEGF while it was 31.2±2.8/HP, and 22.4±2.0/HP in HCC negative for iNOS and VEGF respectively ( P <0.01). A correlation between NOS expression and VEGF in HCC was not observed.Conclusion iNOS and eNOS may play a role in malignant transfornation of post hepatic cirrhosis. The expression of iNOS and VEGF favors angiogenesis of HCC.

关 键 词：肝肿瘤 肝细胞 NDS VEGF 肿瘤血管形成 

分 类 号：R735.7[医药卫生—肿瘤]