改进原位接种技术建立裸小鼠人大肠癌(SW1116)模型  

A New Orthotopic Transplantation Technique for Establishing Human Colon Cancer SW1116 Model in Nude Mice

作　　者：鱼达[1] 余海 陈丽荣[2] 陈培辉 王海军 

机构地区：[1]医学院实验动物中心,杭州310031 [2]医学院第二附属医院,杭州310009

出　　处：《上海实验动物科学》2000年第2期97-100,共4页Shanghai Laboratory Animal Science

摘　　要：为改进裸小鼠肠壁原位肿瘤接种方法和提高肿瘤转移发生 ,用自创的肿瘤肠壁粘接和脾切除技术 ,在裸小鼠体内筛选并建立稳定的 ,有转移能力的模型。结果发现 ,对裸小鼠皮下接种后生长的无转移能力的人 SW1 1 1 6大肠癌组织块进行大部切除 ,可诱发肿瘤转移 ;用肿瘤肠壁原位粘接技术可获得肿瘤的自发转移个例 ,同时使用脾切除技术更可获得肿瘤的高转移。结果提示 ,人大肠癌的转移能力可在裸小鼠体内诱导增强 ;To establish a human colon cancer model in nude mice, a new anastomotic orthotopic transplatation technique was developed. Human colon cancer SW1116 cells were implanted subcutaneosly and passaged in nude mice for 4 generations ,no metastasis was found. From 5th generation the tumors were resected subtotally when reaching 1.5 cm in diameter. After the tumor grew again,1 of the 8 mice appeared metastatic lymph node in the armpit of the forelimb. A medical adhesive, OB Glue, was used in orthotopic transplantation of the intact SW1116 tumors from the metastatic lymph node. At the first 4 passages the metastatic rate was low. From the 5th passage, the spleen of mice were also resected at the time of tumor orthotopic transplantation, with which the metastatic rate was high. From 6th to 10th passage the metastatic rate was 100%(40/40) to the mesenteric lymph nodes, 75%(30/40) to the livers. It can be considered that the metastatic SW1116 sub population (subpop) was obtained. The orthotopic Transplantation method and SW1116 subpop model of nude mice may be useful for further study of human colon cancer.

关 键 词：大肠癌 裸小鼠模型 原位接种 自发性转移 

分 类 号：R735.34[医药卫生—肿瘤] R－332[医药卫生—临床医学]