改良血管阻塞方法建立大鼠全脑缺血模型  

Modified Vascular Occlusion to Establish Cerebral Global Ischemia Model

作　　者：严智文[1] 董河[1] 冯伟[1] 牛泽军[1] 褚海辰[1] 隋爱华[2] 

机构地区：[1]青岛大学医学院,山东青岛266003 [2]青岛大学医学院附属医院

出　　处：《中国医学创新》2013年第1期1-3,共3页Medical Innovation of China

基　　金：山东省自然科学基金(编号:Y2006C14)

摘　　要：目的:探讨改良椎动脉阻断法建立四血管阻塞全脑缺血模型的可行性。方法:健康成年雄性Wistar大鼠68只,随机分为假手术组(S组)8只、传统模型组(T组)及改良模型组(I组)各30只。传统组采用三步制模法:第1步暴露第1颈椎双翼孔,在显微镜下磨钻暴露椎动脉后用双极电凝器烧闭双侧椎动脉;第2步游离双侧颈总动脉,放入线扣;第3步24h后夹闭双侧颈总动脉,建立全脑缺血再灌注模型;改良组在第1步用自制改良电烙铁直接插入翼孔将椎动脉烧闭,其余步骤同传统组;假手术组不凝闭椎动脉和不夹闭颈总动脉。观察改良组和传统组模型制作耗费时间、成功率、操作复杂度、设备费用。比较改良组、传统组与假手术组在行为学和病理学上的差异。结果:与传统组比较,改良组模型制作时间明显减少(P<0.05),成功率明显提高,设备费用大幅减少。HE染色及Morris水迷宫显示模型组与假手术组在病理学和行为学比较差异有统计学意义(P<0.01)。结论:改良组与传统组均成功制模,改良后的制作方法成功率高,制模时间短,无需特殊设备,方法简便可靠。Objective：To explore the feasibility of modified vertebral artery occlusion to establish global cerebral ischemia rat model.Method：48 adult healthy male wistar rats were randomly divided into three groups： the sham operation group（group S, n=8）,the traditional group（group T,n=30） and the improved group（group I,n=30）.The rats from group T were done by three steps.First, we enlarged the alar foramina of the first cervical vertebra through which the vertebal arteries travelled with the grinding drill in order to visualize the vertebral arterises sufficiently and then coagulated the vertebral arteries by the microscope.Second,bilateral common cervical artery were dissociated and threaded. Finally,the bilateral common cervical artery were clamped for 10 minutes after 24 hours.The rats from group I accepted the same procedure as rats from group T except the bialteral vertebral arteries were interdicted with the electric iron which made by ourself.The rats from group S accepted the same procedure as the rats from group T except that the vertebral arteries were not interdicted and the bialteral vertebral arteries were not clamped. Both model groups were assessed with fees、the ratio of success and preparation time and costs.The group S was compared with the group I and T in ethology and pathology.Result：Compared with group T,the modified surgical approach can increase the successful ratio and reduce preparation time and costs（P0.05）.HE and Morris showed that group T and I had significant difference with group S in pathology and behavior（P0.01）.Conclusion：The group T and I were successful,but cerebral ischemia model with modified artery occlusion is more simple and reliable.

关 键 词：全脑缺血模型 四血管阻塞法 椎动脉 

分 类 号：R-332[医药卫生]