NGF-Tf偶联物对PD模型中黑质神经元的保护作用  

NGF-Tf Conjugate Prevents Degeneration of Substantia Nigra Neurons in a Mouse Model of Parkinson's Disease

作　　者：李晓飙[1] 廖共山[1] 黄绍明 舒雨雁[1] 汤圣希[1] 

机构地区：[1]广西医科大学蛇毒研究所,南宁530021

出　　处：《生物化学与生物物理学报》2000年第4期413-416,共4页

基　　金：广西壮族自治区科委资助项目!No.98110 3 9;9912 0 2 7&&

摘　　要：中华眼镜蛇毒纯化的神经生长因子 (NGF)与转铁蛋白 (Tf)通过生物素 亲和素偶联 ,观察其对于 1 甲基 4 苯基 1,2 ,3,6 四氢吡啶 (MPTP)引发的小鼠帕金森病 (PD)模型中黑质神经元的保护作用。电镜下对小鼠脑组织切片的观察表明 :MPTP组神经元的髓鞘脱落 ,并出现大量空泡和线粒体嵴变形 ;光镜下对HE染色的切片则观察到MPTP组神经元中出现空泡 ,Nissl体溶解 ,胞核固缩 ,并有Lewy小体形成 ,同时伴有血管破裂 ,而在NGF组及正常对照组并未发现上述病理改变 ;在免疫组化实验中 ,对黑质部位的多巴胺细胞计数结果为 :MPTP组797.0± 12 1.4,NGF组 2 330 .0± 2 6 0 .3,正常对照组 2 381.0± 15 8.0 ,经统计检验表明 ,NGF组细胞存活数显著高于MPTP组 (P <0 .0 5 ) ,而NGF组与正常对照组的细胞存活数不存在显著性差异 (P >0 .0 5 )。Nerve growth factor(NGF) was purified from Naja naja atra snake venom and conjugated to transferrin(Tf). This conjugate was intravenously injected into a mouse model of Parkinson's disease (PD). Both immunohistochemical staining and pathological detection showed that the NGF Tf conjugate could prevent the loss of tyrosine hydroxylase immunoreactive neurons located in substantia nigra and the cell counts of NGF group were 2 330.0±260.3, and those of the MPTP group and the control group were 797.0±121.4 and 2 381.0 ±158.0, respectively. In addition, electron microscopic examination revealed significant protection against demyelination and vacuolation in subtantia nigra neurons in contrast to the control group. The i.v. injected NGF Tf conjugate also reversed the neurodegenerative changes such as karyopyknosis, chromatolysis and intracytoplasmic inclusion in diseased neurons.

关 键 词：神经生长因子 TF 帕金森病 黑质神经元 治疗 

分 类 号：R742.5[医药卫生—神经病学与精神病学]