研磨法和组织分离器制备乳腺癌腋窝淋巴结单细胞悬液的比较  

Comparison of grinding method and tissue separator in the preparation for the single cell suspensions of breast cancer axillary lymph nodes

作　　者：陈相彪[1] 杨伟明[1] 陆婧[1] 王春林[1] 唐聃[1] 贺新媛[1] 骆礼波[1] 石磊[1] 

机构地区：[1]遵义医学院附属医院甲状腺乳腺外科,贵州遵义563099

出　　处：《遵义医学院学报》2012年第5期435-437,共3页Journal of Zunyi Medical University

基　　金：贵州省教育厅重点项目(NO:黔教科(2009)0110)

摘　　要：目的比较研磨法和Gentle MACS组织分离器制备乳腺癌患者腋窝淋巴结单细胞悬液在活细胞率、丝状物及细胞团块方面的优缺点,为淋巴组织细胞培养和流式分析找到一种更为可靠的方法。方法分别用研磨法和Gentle MACS组织分离器制备单细胞悬液,台盼兰染色后计数活细胞、死细胞、细胞团块和丝状物,并比较两种方法的优缺。结果两种方法制备的腋窝淋巴组织单细胞悬液在细胞存活率方面有显著性差异(P<0.05)。在丝状物和细胞团块方面,组织分离器法丝状物和细胞团块数量更少(P<0.05)。结论制备淋巴组织单细胞悬液,组织分离器法较研磨法更为优异。Objective To compare the grinding method and Gentle MACS tissue separator in the prepa- ration for the single cell suspensions of axillary lymph nodes in breast cancer and further find a more re- liable method for cell culture and flow eytometrie analysis of lymphoid tissues. Methods The single cell suspensions were prepared using the grinding method and Gentle MACS tissue separator. The numbers of living cells, dead cells, cell clumps and filaments were counted through trypan blue staining to further investigate the advantages and disadvantages of these two methods. Results No significant difference of cell viability between grinding method and Gentle MACS tissue separator was shown. However, compared with the grinding method, fewer filaments and cell clumps were discerned via Gentle MACS tissue separator. Conclusion Tissue separator might be better than grinding method in the preparation for lymph node single cell suspensions.

关 键 词：乳腺癌 淋巴组织 单细胞悬液 

分 类 号：R737.9[医药卫生—肿瘤]