3种抗肝癌药物单用和联用对人肝癌细胞BEL7402和人正常肝细胞增殖的抑制作用  

Roles of cisplatin,fluorouracil,or daunorubicin alone or in combination for inhibition of liver carcinoma cell line BEL-7402 and normal human liver cells

作　　者：肖亿[1] 

机构地区：[1]广西柳州市人民医院,545005

出　　处：《实用医学杂志》2013年第1期21-23,共3页The Journal of Practical Medicine

基　　金：广西卫生厅自筹基金课题(编号:Z2010056)

摘　　要：目的:观察DDP、5-Fu、EADM在单用、两两联用和3药联用时对人肝癌细胞株BEL7402和人肝细胞增殖的抑制作用。方法:MTT法测定3种药物对人肝癌细胞株BEL7402和人肝细胞增殖的抑制率,绘制生长曲线。结果:单用时3种药对BEL7402的抑制率分别为49.55%、31.46%、39.87%;对肝细胞的抑制率分别为27.32%、19.66%、21.58%;DDP+5-Fu对BEL7402的抑制率为72.39%,对肝细胞的抑制率为34.89%;DDP+EADM对BEL7402的抑制率为56.42%,对肝细胞的抑制率为32.46%;5-Fu+EADM对BEL7402的抑制率为67.92%,对肝细胞的抑制率为37.12%;3药联用对BEL7402的抑制率为86.93%,对肝细胞的抑制率为41.69%。结论:3种药物单药、两两联用和3药联用均对人肝癌细胞株BEL7402有明显的增殖抑制作用,同时可抑制人肝细胞的增殖。Objective To explore the anti-proliferative effect of cisplatin （DDP）, fluorouracil （5-FU）, and EADM ahme or in combination for liver carcinoma cell line BEL-7402 amt human liver cells. Methods MTF assay and the cell growth curve were used to determine the inhibitory effect. Results The inhibitory rate of DDP, 5-FU, or EADM alone was 49.55%, 31.46%, and 39.87% for BEL-7402 and 27.32%, 19.66%, and 21.58% tor normal live cells ; the inhibitory rate of DDP plus 5-FU was 72.39% and 34.89%, respectively. The inhibitory rate of DDP plus EADM was 56.42% and 32.46%. The inhibitory rate of 5-FU plus EADM was 67.92% and 37.12%. The inhibitory rate of three antitumor drugs in combintion was 86.93% and 41.69%. Conlusions DDP, 5-FU, or EADM alone, or two or three drugs in combination has a marked inhibitory effect on proliferation of both liver carcinoma cell line BEL-7402 and normal human liver cells.

关 键 词：DDP 5-Fu EADM 人肝癌细胞BEL7402 肝细胞 增殖抑制 MTT法 体外 

分 类 号：R735.7[医药卫生—肿瘤]