JNK/MAPK信号通路在咖啡酸诱导人白血病K562细胞凋亡中的作用  

Role of JNK/MAPK in caffeic acid-induced apoptosis of leukemia K562 cells

作　　者：莫清华 殷清华 

机构地区：[1]湖南省衡阳市第一人民医院,421001 [2]湖南省岳阳市二人民医院,414000

出　　处：《实用医学杂志》2013年第1期26-28,共3页The Journal of Practical Medicine

摘　　要：目的:探讨c-Jun氨基末端激酶/促分裂原活化蛋白激酶(JNK/MAPK)信号通路在咖啡酸(caffeic acid)诱导人白血病K562细胞凋亡中的作用。方法:体外培养人白血病K562细胞,采用噻唑蓝比色法(MTT)检测细胞的存活率;采用荧光显微镜观察和PI染色流式细胞术检测细胞凋亡率;采用Westernblot方法检测磷酸化JNK/MAPK蛋白、JNK/MAPK蛋白的表达。结果:咖啡酸能明显抑制K562细胞的生长,且可诱导K562细胞凋亡,JNK/MAPK磷酸化的激活。在予以SP600125(JNK/MAPK特异性抑制剂)预处理后,咖啡酸抑制K562细胞生长的作用明显减弱,凋亡细胞减少,细胞凋亡率下降,JNK/MAPK活性显著下降。结论:咖啡酸可诱导K562细胞凋亡,其作用机制可能通过激活JNK/MAPK。Objective To determine the role of JNK/MAPK signal transduction pathways in caffeic acid- indue.ed apoptosis in leukemia K562 cells. Methods K562 cells growth inhibition was measured by MTF assay. Apoptosis of K562 cells was studied by AO/EB fluorescence stain and flow cytometry with PI staining. Expression of JNK, phosphorylation of JNK （p-JNK） MAPK were determined by Western blot analysis. Results Caffeie acid eould induce apoptosis of K562 eells and the activity of p-JNK in K562 cells was apparently increased. However, after treatment with JNK/MAPK inhibitor （SP600125）, the inhibitory effect of caffeie acid on K562 cells was significantly weakened, the apoptotic rate of the ceils and the activity of p-JNK also decreased dramatically. Conclusions Caffeic acid can induce apoptosis of K562 cells, and JNK inhibitor ean significantly inhibit the apoptosis induced by caffeic acid in K562 cells. Cafleic acid induced apoptosis of K562 cells involves the activation of JNK/MAPK.

关 键 词：白血病 咖啡酸 人白血病K562细胞 细胞凋亡 JNK MAPK 

分 类 号：R733.7[医药卫生—肿瘤]