β-环糊精增敏荧光法测定果蔬中呋喃丹的残留量  

Fluorometric determination of carbofuran residues in fruits and vegetables with β-CD sensitizer

作　　者：李满秀[1] 姚旭梅[1] 冯娜[1] 许妍[1] 

机构地区：[1]忻州师范学院化学系,忻州034000

出　　处：《分析试验室》2013年第1期61-63,共3页Chinese Journal of Analysis Laboratory

基　　金：山西省高等学校教学改革项目基金(J2012091);山西省大学生创新创业训练项目基金(2012334)资助

摘　　要：利用荧光光谱法探讨了β-环糊精与呋喃丹间的超分子相互作用,证明β-环糊精与呋喃丹可形成1:1的超分子包合物,在λex/λem=272/323 nm处发射强荧光,包合常数为108.2 L·mol-1。据此建立了测定水溶液中呋喃丹的荧光分析新方法。在0.0025～0.03μg/mL范围内,呋喃丹的荧光强度与浓度呈良好的线性关系,相关系数R=0.9990,方法的检出限为0.0013μg/mL,相对标准偏差为1.2%。该体系的抗干扰能力及稳定性好,实际样品分析回收率为98%～102%。The supramolecular interaction between carbofuran and β-cyclodextrin was studied by spectrofluorimetry.The results show that β-cyclodextrin reacts with carbofuran to form an inclusion complex with an association constant of 108.2L/mol.The composition of the complex is 1：1（β-CD： carbofuran）.Based on the significant enhancement of fluorescence intensity of carbofuran in the inclusion complex,a spectrofluorimetric method with high sensitivity and selectivity was developed for the determination of carbofuran in aqueous solutions.Under the optimum conditions,the complex had maximum excitation and emission wavelengths at 272 nm and 323 nm,respectively.The linear range of the method was 0.0025-0.030 μg/mL with a detection limit of 0.0013 μg/mL,and the relative standard deviation was 1.2%.The recoveries of the determination were 98%-102%.

关 键 词：Β-环糊精 增敏荧光法 呋喃丹 

分 类 号：O657.3[理学—分析化学]