刺五加Cu/Zn SOD的克隆及内生真菌对其表达的影响  

Copper/Zinc Superoxide Dismutase Gene Cloning in Eleutherococcus senticosus and Its Expression by Endophytic Fungus

作　　者：邢朝斌[1] 龙月红[1] 李宝财[1] 何闪[1] 梁能松[1] 朱金丽[1] 

机构地区：[1]河北联合大学,唐山063000

出　　处：《东北林业大学学报》2013年第1期82-85,105,共5页Journal of Northeast Forestry University

基　　金：国家自然科学基金项目(30701086);河北省自然科学基金项目(C2009001252);河北省自然科学基金-石药集团医药联合研究基金项目(H2012401006)

摘　　要：利用RACE技术从刺五加(Eleutherococcus senticosus Harms)中克隆到铜锌型超氧化物歧化酶基因(Cu/Zn SOD)的cDNA序列。获得的刺五加Cu/Zn SOD的cDNA长641 bp。基因内部含有1个长度为459 bp的开放阅读框,编码长度为152个氨基酸残基的蛋白质,预测分子质量为15.162 ku,理论等电点为5.29。刺五加Cu/Zn SOD氨基酸序列与其它植物的Cu/Zn SOD具有很高的相似性。相对定量RT-PCR的结果表明,回接内生真菌菌株P116-1a、P116-1b、P109-4和P312-1后可显著提高刺五加Cu/Zn SOD基因的表达量(P<0.05),最大表达量出现在菌株P312-1回接60 d时,达对照的4.99倍。The full length cDNA of Copper/Zinc Superoxide Dismutase gene （ Cu/Zn SOD ） in Eleutheroeoccus senticosus was first cloned by Rapid Amplification of cDNA Ends （RACE） method. The full length cDNA of Cu/Zn SOD in E. senticosus is 641 bp, containing an open reading frame （ORF） of 459 bp. The ORF encodes a protein with 152 amino acids. The predicted molecular quantity of the protein is 15. 162 ku, and the theoretical iso-electric point is 5.29. The amino acid se- quence of E. sentieosus Cu/Zn SOD is highly similar with amino acid sequence of other plants. Relative quantification re- sults obtained by RT-PCR show that the expression of Cu/Zn SOD is significantly improved by wound back endophytic fun- gus Pll6-1a, Pll6-1b, P109-4 and P312-1 （P〈0.05）. The highest expression amount of Cu/Zn SOD appeares when P312-I wounded back after 60 days, and the amount is 4.99 times more than that of the control.

关 键 词：刺五加 铜锌超氧化物歧化酶 克隆 内生真菌 

分 类 号：S759.82[农业科学—森林经理学] Q785[农业科学—林学]