高效液相色谱法测定小麦粉和大米粉中的氯啶菌酯  

Determination of Chloropiperidine Ester in Wheat Flour and Rice Flour by HPLC

作　　者：张月[1] 吕岱竹[1] 林静凌[1] 李建国[1] 

机构地区：[1]中国热带农业科学院分析测试中心,海口571101

出　　处：《农药》2013年第1期48-49,66,共3页Agrochemicals

基　　金：海南省自然基金(211018);海南省自然基金(311060)

摘　　要：[目的]建立小麦粉和大米粉中氯啶菌酯的液相色谱检测方法。[方法]样品经乙腈提取,过滤,C18柱净化。采用高效液相色谱检测,色谱柱:SunFire C18柱;流动相:乙腈-水(体积比70:30);流速:1.0 mL/min;检测波长:234 nm;进样量:10μL。[结果]该方法在小麦粉和大米粉中添加0.05、0.1、0.5 mg/kg三个不同水平,回收率分别为77.6%～103%和92.2%～99.6%。[结论]该方法满足农药残留检测的要求。[Aims] The high performance liquid chromatography （HPLC） method was established for analyzing the chloropiperidine ester in wheat flour and rice flour. [Mothods] Samples were firstly extracted by acetonitrile and then homogenized, filtrated, finally purified by C18 column. The experiment was carried on SunFire C18 column with the mobile phase of acetonitrile-water （70 ： 30, by vol） at the flow rate of 1.0 mL/min. The detection wavelength and injection volume were set at 234 nm and 10 μL respectively. [Rosults] The recoveries of three different concentration （0.05, 0.1, 0.5 mg/kg） for chloropiperidine ester were between 77.6 to 103% and 92.2 to 99.6%, respectively. [Conclusions] This method met with the requirements of the pesticide residue detection.

关 键 词：氯啶菌酯 小麦 大米 高效液相色谱 

分 类 号：TQ450.263[化学工程—农药化工]