两种滇产木姜子属植物中总黄酮的抗氧化活性研究  

Study on the Antioxidant Activities of Total Flavonoids from Two Plants of Litsea in Yunnan

作　　者：王少杰[1] 马水仙[1] 张娜[1] 周树娅[1] 尹艳清[1] 杨丽娟[1,2] 

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]云南民族大学云南省聚乳酸基功能材料工程实验室,云南昆明650500

出　　处：《亚太传统医药》2013年第1期19-21,共3页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金项目(21162042);云南自然科学基金项目(2010CD090);云南省高校化学类核心课程教学团队项目

摘　　要：目的:对滇产金平木姜子和红叶木姜子进行总黄酮含量的测定,并分别研究总黄酮的抗氧化活性。方法:用正丁醇分别浸提金平木姜子和红叶木姜子得到两种提取物,用紫外分光光度法测定各自总黄酮的含量,同时以DPPH清除率实验来评价其抗氧化活性并分别绘制DPPH随浓度变化曲线进行比较。结果:金平木姜子中黄酮提取物的DPPH自由基清除率最高为72.63%,IC50值为1.75mg/mL;红叶木姜子中黄酮提取物的DPPH自由基清除率最高为86.67%,IC50值为1.48mg/mL。结论:金平木姜子和红叶木姜子中含较丰富的总黄酮化合物,具有较强的抗氧化活性,且红叶木姜子中总黄酮的抗氧化活性高于金平木姜子。Objective：To determine the total flavonoids in Litsea chingpingensis and Litsea rubescens from Yunnan and investigate their antioxidant activities of DPPH. Methods： The flavonoids were extracted from the two plants of Litsea with n-butanol. Their contents were determined by UV-Vis spectrophotometry and the antioxidant activities were evaluated by the removal rate for DPPH. For further investigation, the standard curve could be drawn by using these data. Results：The DPPH removal rate of flavonoids in Litsea chingpingensis was 72.63 ; and its IC50 value was 1.75 mg/mL. While the DPPH removal rate of flavonoids in Litsea rubescens was 86. 67 ; and its IC50 value was 1.48mg/mL. Conclusion：The Litsea chingpingensis and Litsea rubescens were riched in flavonoids which had good antioxidant activities, and the antioxidant activity of Litsea rubescens is superior to that of Litsea chingpingensis.

关 键 词：金平木姜子 红叶木姜子 总黄酮 DPPH 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]