小鼠胚胎腭突融合过程中IRF6蛋白在腭突组织中表达的实验研究  

Experimental study of IRF6 protein expression in palatal tissues during embryonic palatine process fusion in mice

作　　者：周容[1] 陈秀娟[1] 肖文林[1] 钟凤[1] 

机构地区：[1]青岛大学医学院附属医院口腔科,山东青岛266071

出　　处：《重庆医学》2013年第2期124-126,共3页Chongqing medicine

基　　金：国家自然科学基金资助(81070817);青岛市科技计划项目资助(08-2-1-5)

摘　　要：目的观察小鼠胚胎腭突融合过程中IRF6蛋白在腭突组织中的表达情况,探讨唇腭裂发生原因。方法 60只C57BL/6J近交系受孕小鼠分别于妊娠第13.0天(GD13.0)、GD14.0、GD14.5及GD15.0时断颈处死,取小鼠胚头经固定、石蜡包埋、切片,行苏木素-伊红染色及免疫组织化学检测不同胚胎发育时期的腭突组织中IRF6蛋白的表达。结果 GD13.0时,腭突上皮、舌上皮可见IRF6蛋白表达;GD14.5时,腭突中嵴上皮接触,IRF6蛋白的表达水平达到高峰;GD15.0时,表达水平下降(P<0.05)。结论 IRF6蛋白在两侧腭胚突的接触、融合期发挥调节作用。Objective To observe IRF6 protein expression in palatal tissues during mice embryonic palatine process fusion and to explore pathogenesis of deft lip and palate. Methods Sixty C57BL/6J inbred and conceive mice were sacrificed by having their necks broken on gestation day 13.0 （GD13.0）, GD14.0, GD14.5 and GD15.0. The mice embryo heads were fixed, paraffin embedded,coronal sectioned. Hematoxylin-eosin staining and immunohistochemistry were performed to detect IRF6 protein expression in palatal tissues in different embryonic development period. Results IRF6 protein expression was found in palatine process epithelia, tongue epithelia on GD13.0, and reached the peak on GD14.5 when the palatal medial edge epithelia contacted,and decreased on GD15.0（P〈0.05）. Conclusion IRF6 protein play a regulatory role in periods of bilateral palatine process contact and fusion.

关 键 词：胚胎发育 小鼠 近交系 腭裂 干扰素调节因子6 腭突融合 

分 类 号：R782.2[医药卫生—口腔医学]