依维莫司联合顺铂对人胃癌SGC7901细胞凋亡及Bcl-2、Bax表达的影响  

Effects of everolimus combined with cisplatin on apoptosis and Bcl-2,Bax expression of human gastric cancer SGC7901 cells

作　　者：刘莹[1] 朱祖安[2] 崔涛[1] 孔庆兖[1] 刘磊[1] 张瑞瑞[1] 

机构地区：[1]徐州医学院病理学教研室,江苏徐州221004 [2]徐州医学院附属医院消化内科,江苏徐州221002

出　　处：《重庆医学》2013年第2期138-142,共5页Chongqing medicine

摘　　要：目的探讨依维莫司(RAD001)联合顺铂对人胃癌SGC7901细胞凋亡及Bcl-2、Bax表达的影响。方法体外培养SGC7901细胞,将其分为对照组,顺铂组(顺铂2.50 mg/L),RAD001低浓度组(RAD001 5.00nmol/L)、RAD001中浓度组(RAD001 10.00nmol/L)、RAD001高浓度组(RAD001 20.00nmol/L)及联合组(顺铂1.25mg/L+RAD001 5.00nmol/L),采用流式细胞术检测SGC7901细胞的凋亡率,采用免疫细胞化学、Western blot检测SGC7901细胞Bcl-2和Bax的表达情况。结果 RAD001、顺铂作用SGC7901细胞48h后,对照组,顺铂组,RAD001低、中、高浓度组及联合组的细胞凋亡率分别为(8.04±0.48)%、(18.94±0.75)%、(10.47±1.05)%、(13.93±2.45)%、(17.20±0.65)%及(23.18±1.05)%。除RAD001低浓度组细胞凋亡率与对照组比较,差异无统计学意义(P>0.05),其他各组细胞凋亡率均较对照组显著增加(P<0.05)。与对照组比较,顺铂组、RAD001组及联合组SGC7901细胞Bcl-2蛋白表达下降,而Bax蛋白表达增加,Bcl-2/Bax比值降低(P<0.05),联合组改变最明显。结论 RAD001联合顺铂可通过上调Bax和下调Bcl-2蛋白的表达诱导SGC7901细胞凋亡。Abstract：Objective To investigate everolimus（RAD001） combined with cisplatin on apoptosis and Bcl-2, Bax expression of human gastric cancer SGC7901 cells. Methods SGC7901 cells were cultured in vitro and divided into control group,cisplatin group （cisplatin 2.50 mg/L）,RAD001 low concentration group（RAD001 5.00 nmol/L）,RAD001 medium concentration group（RAD001 10.00 nmol/L）, RAD001 high concentration group （RAD001 20. 00 nmol/L） and combination group （cisplatin 1. 25 mg/L-＋ RAD001 5.00 nmol/L）. Flow cytometry was employed to detect apoptotic rate of SGC7901 cells and immunocytochemistry and Western blot were conducted to measure Bcl-2,Bax expression of SGC7901 cells. Results After 48 h treatment,apoptotic rates of SGC7901 cells in control group, cisplatin group, RADO01 low concentration group, RAD001 medium concentration group, RAD001 high concentration group and combination group were （8.04 ± 0.48 ） %, （ 18.94 ± 0.75） %, （ 10.47 ± 1.05 ） %, （ 13.93 ± 2.45 ） %, （17.20± 0.65） % and （23. 18± 1.05）%. Except RAD001 low concentration group showing no statistically significant difference when compared to control group, the apoptotic rates in the other groups were obviously increased（P〈0.05）. Compared with con- trol group,SGC7901 cells in cisplatin group,RAD001 groups and combination group showed decreasing Bcl-2 and increasing Bax protein expression,and lower Bcl-2/Bax ratio（P〈0.05）. Changes in combination group were the most obvious. Conclusion RAD001 combined with cisplatin can induce apoptosis in SGC7901 ceils via up-regulating Pax and down-regulating Bcl-2 protein expression.

关 键 词：细胞凋亡 基因 BCL-2 基因 bax 顺铂 依维莫司 

分 类 号：R735.2[医药卫生—肿瘤]