川产柑橘属不同品种成熟果皮中柠檬苦素的含量测定  

Determination of Limonoids in Varieties of Mature Citrus Peel in Sichuan by HPLC

作　　者：彭腾[1] 邱建平[1] 李鸿翔[1] 邓赟[1] 贾波[1] 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,四川成都611137

出　　处：《成都中医药大学学报》2012年第4期36-38,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：成都中医药大学科技发展基金项目(编号:ZRYB200804)

摘　　要：目的:测定川产柑橘属不同品种成熟果皮中柠檬苦素的含量。方法:采用RP-HPLC测定柠檬苦素的含量。色谱柱:Diamonsil C18(250 mm×4.6 mm,5μm);流动相:乙腈:水=42:58;流速:1.0mL/min;柱温:30℃;检测波长:215 nm。结果:柠檬苦素在0.3352～3.2682μg范围内线性关系良好,回归方程为:Y=638412X+41961(r=0.9992),平均回收率为98.27%,RSD为1.79%(n=6)。结论:该方法灵敏、准确,适用于柑橘类成熟果皮中柠檬苦素的含量测定。Objective：To determinate the content of limonoids in varieties of mature citrus peel in Sichuan.Methods：A reversed phase HPLC method was established to determinate the content of limonoids.The samples were analyzed on C18 column（250 mm×4.6 mm,5 μm）,with the mobile phase of acetonitrible-H2O（42：58） at the detection wavelength of 215 nm,the flow rate of 1.0mL·min-1 and column temperature was 30 ℃.Results：The linear range was from 0.3352 to 3.2682μg for limonin with regression equation as：Y=638412X＋ 41961（r=0.9992）.The average recovery was 98.27 % with RSD of 1.79 %（n=6）.Conclusion：It proved that the process is sensitive and accurate,and can be used to determine the contents of limonoids in mature citrus peel.

关 键 词：川产 不同品种 柑橘果皮 柠檬苦素 高效液相色谱 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]