煤基活性炭固定化脂肪酶制备手性苯乙醇  

SYNTHESIS OF CHIRAL PHENYLETHANOL CATALYZED BY IMMOBILIZED LIPASE OF COAL-BASED ACTIVATED CARBON

作　　者：房俊卓[1] 王璐[1] 张立根[1] 薛屏[1] 

机构地区：[1]宁夏大学能源化工重点实验室,银川硕士副教授750021

出　　处：《煤炭转化》2013年第1期75-78,共4页Coal Conversion

基　　金：宁夏自然科学基金资助项目(NZ1049)

摘　　要：在有机溶剂中制备高选择性固定化脂肪酶是获得高对映体纯度的手性药物中间体的关键步骤.探讨了不同类型的活性炭作为固定化酶载体,应用于拆分手性1-苯乙醇反应,用N2吸附法和扫描电镜表征了活性炭载体.以固定化酶催化拆分(R,S)-1-苯乙醇为典型反应,研究了用不同活性炭为载体制备的催化剂的催化活性以及反应效果随反应时间的变化规律.结果表明,以微孔活性炭作载体制备的固定化酶催化活性最好,当反应时间达到12.8h时,转化率达到最大理论转化率50%.ABSTRACT Making immobilized lipase with high selectivity in organic solvent is a critical step in producing chiral intermediate of enzymatic resolution. The research concentrated on the chiral separation reaction of （R,S）-l-phenylethanol catalyzed by immobilized lipase supported by different coal-based activated carbon. The properties of activated carbon were characterized by the N2 adsorption/desorption method and the scanning electronic microscope. By researching the catalytic sepa- ration reaction of （R,S）-l-phenylethanol catalyzed by immobilized lipase, the catalytic activity of lipase with different carrier of activated carbons as well as the reaction effect with reaction time were studied. The result showed that the immobilized lipase supported by microporous activated carbon was excellent for its catalytic effect, whose conversion rate was nearly 50% when the reaction time reached 12.8 h. KEY WORDS coal-based activated carbon, immobilized lipase, chiral, 1-phenylethanol

关 键 词：煤基活性炭 固定化脂肪酶 手性 1-苯乙醇 

分 类 号：TQ424.11[化学工程]