牡丹反转录转座子RT基因的分离与序列分析  

Isolation and Characterization of Ty1-copia Retrotransposon Sequence from Paeonia suffruticosa Andrews

作　　者：贾甜甜[1] 郭大龙[2] 侯小改[1] 刘改秀[3] 王丽娜[3] 张修铭[2] 李双双[2] 

机构地区：[1]河南科技大学农学院,河南洛阳471003 [2]河南科技大学林学院,河南洛阳471003 [3]中国洛阳国家牡丹基因库,河南洛阳471006

出　　处：《华北农学报》2012年第6期30-33,共4页Acta Agriculturae Boreali-Sinica

基　　金：河南省高校科技创新人才支持计划项目(2010HASTTT002);国家自然科学基金(31070620);河南省高等学校青年骨干教师项目(2010GGJS-072);河南科技大学大学生研究训练计划(2011270)

摘　　要：以牡丹基因组为模板,利用简并引物进行PCR扩增,得到了编码Ty1-copia类反转录转座子反转录酶(RT基因)的序列,测序后所得序列长度为240 bp,包含2个开放读码框,共编码63个氨基酸。经NCBI中BLAST比对结果显示,洛阳红牡丹RT基因与枸杞、甜菜等RT基因的同源性均在70%以上。经MEGA和DNAstar软件分析表明,该片段在进化上与梅、杨、茶和苹果等落叶木本植物的同源性序列存在更高的同源性和更近的亲缘关系,与枸杞、鹰嘴豆、番茄、草莓等草本植物的同源性序列亲缘性较远。With degenerate primer,the Ty1-copia-like retrotransposon sequence fragments were amplified from peony（Paeonia suffruticosa Andrews.）genomic DNA and sequemced,getting a 240-bp PCR fragment which contains two open reading frames and encodes 63 amino acids.The results of BLAST in GenBank showed that the RT gene sequence of peony had more than 70% similarity with those of Lycium chinense and Beta vulgaris,etc.A phylogenetic analysis by MEGA and DNAstar software showed that the sequence had higher homology and closer paternity with those of deciduous woody trees such as Prunus mume,Populus ciliate,Camellia sinensis and Malus x domestica than those of herbaceous plants such as L.barbarum,Cicer arietinum,Lycopersicon esculentum and Fragaria viridis.

关 键 词：牡丹 反转录转座子 RT基因 克隆 

分 类 号：S685.11[农业科学—观赏园艺]