核桃分心木化学成分与生物活性研究  

Chemical Constituents and Bioactivity Studies of Diaphragma juglandis Fructus

作　　者：杨明珠[1] 田新雁[1] 肖朝江[1] 韩冰洋[1] 姜北[1] 

机构地区：[1]大理学院药学院,大理671000

出　　处：《天然产物研究与开发》2012年第12期1707-1711,1728,共6页Natural Product Research and Development

基　　金：云南省中青年学术技术带头人后备人才项目(2008PY005);教育部留学回国人员科研启动基金[2009]1001

摘　　要：利用色谱分离方法与现代波谱分析技术,对核桃分心木的化学成分进行了系统的研究。由其醇提物乙酸乙酯部位分离鉴定了11个化合物,分别为大黄素(1)、齐墩果酸(2)、Isosclerone(3)、正十七烷(4)、3-羟基-1-(4-羟基取代苯基)-1-丙酮(5)、硬脂酸甘油单酯(6)、没食子酸(7)、没食子酸乙酯(8)、核桃素D(9)、对羟基苯甲酸(10)、香草酸(11)。运用DPPH(1,1-二苯基苦基苯肼)法、纸片扩散法、肉汤稀释法对分心木粗提物及各相关样品的抗氧化与抗菌活性进行了初步研究。结果显示,分心木提取物及各相关样品普遍具有较好的抗氧化与抗菌活性。分心木具有多种活性物质,具有一定的药用与开发价值。Based on chromatographic separation and modem spectroscopy analysis techniques, eleven compounds were separated and purified from the ethyl acetate fraetion of the 75% ethanol extract of Diaphragma juglandis Fmctus. The structures of the eleven compounds were elucidated as emodin （ 1 ）, oleanolic acid （ 2 ） , isoselemne （ 3 ）, heptadecane （4）, 3-hydroxy-1- （ 4-hydmxyphenyl ） -1-propanone （ S ）, glyceml-l-oetadecanoate （ 6 ）, gallic aeid（ 7 ）, ethyl gallate （8） ,juglanin D （9） ,p-hydmxybenzoic acid （10） and vanillie acid （11）. The antibacterial activities of the fractions ex- tracted from Diaphragma juglandis Fmctus were evaluated by disk diffusion method and routine broth dilution method. In addition, the antioxidant activities of the crude ethanol extract of Diaphragma jug, landis, as well as some sub-fractions of the crude extract, were tested by using DPPH （1,1-diphenyl-2-pierylhydrazyl） method. As a result, most samples showed strong antibacterial and antioxidant activities. The antioxidant activity of ethyl acetate fraction was even stronger than the positive control,vitamin C. The study presented in this paper indicated that Diaphragma juglandis Fmetus con- tains various bioaetive constituents and possesses potential values for medical purposes and application developments.

关 键 词：分心木 化学成分 抗氧化活性 抗菌活性 

分 类 号：R284.2[医药卫生—中药学]