洛伐他汀发酵培养基的优化  

Optimization of Fermentation Medium for Lovastatin Production

作　　者：包良[1] 杨德志[2] 班楠[2] 阿木日沙那[1] 

机构地区：[1]呼和浩特职业学院,内蒙古呼和浩特010051 [2]内蒙古大学生命科学学院

出　　处：《包头医学院学报》2012年第6期8-9,共2页Journal of Baotou Medical College

摘　　要：目的:通过摇瓶发酵筛选出土曲霉(A.terreus)ATCC 20542生物合成洛伐他汀所需的最适碳源和氮源。方法:保持发酵培养基中其他组分不变,分别以麦芽糊精、葡萄糖、乳糖、淀粉作为碳源,以蛋白胨、酵母粉、硫酸铵、大豆粉为氮源进行发酵,并以乙酸乙酯萃取发酵液中的洛伐他汀,用高效液相色谱法(HPLC)对其进行相对定量。结果:四种碳源中,淀粉产生的洛伐他汀的量最大;四种氮源中,大豆粉产生的洛伐他汀的量最大。结论:土曲霉生产洛伐他汀的培养基中最佳碳源为淀粉,最佳氮源为大豆粉。Objective ： To screen the optimal carbon and nitrogen sources of fermentation medium for lovastatin produced by Aspergillus terreus ATCC 20542. Method： With other components of the medium unchanged, maltodextrin, glucose, lactose, starch were tested as the carbon sources, while peptone, yeast powder, ammonium sulfate, soybean powder were tested as the ni- trogen sources. Lovastatin was extracted from the broth by acetoacetate and was measured by high performance liquid chromatog- raphy （HPLC）. Result： More lovastatin was produced when starch and soybean powder were used as the carbon and nitrogen sources, respectively. Conclusion： In fermentation medium for lovastatin produced by Aspergillus terreus, starch was the best carbon source, and soybean powder was the best nitrogen source.

关 键 词：洛伐他汀 发酵 培养基 

分 类 号：R972.6[医药卫生—药品]