转双价基因(Bt+CpTI)棉种植对根际土壤微生物群落功能多样性的影响  

Effects of Transgenic Bt+CpTI Cotton Planting on Functional Diversity of Rhizosphere Soil Microbial Community

作　　者：刘红梅 赖欣 宋晓龙 张海芳 杨殿林 

机构地区：[1]农业部环境保护科研监测所/农业部转基因生物生态环境安全监督检验测试中心(天津),天津300191

出　　处：《中国农学通报》2012年第36期231-236,共6页Chinese Agricultural Science Bulletin

基　　金：转基因生物新品种培育重大专项"自然生态风险监测与控制技术"(2011ZX08012-005);"转基因棉花环境安全评价技术"(2011ZX08011-002);中国农业科学院院长基金"转Bt基因抗虫棉对AM真菌的影响研究"(201020)

摘　　要：为了研究转基因棉花种植对其根际土壤微生物群落功能多样性的影响,采用Biolog方法对不同生育期转双价(Bt+CpTI)基因棉SGK321及其亲本常规棉‘石远321’根际土壤微生物的单一碳源利用水平进行比较分析。结果表明,苗期、蕾期转双价棉土壤微生物群落平均吸光值(AWCD)显著高于常规棉,而花铃期、吐絮期显著低于常规棉。2种棉花根际土壤微生物群落丰富度指数(H)和优势度指数(D)随棉花生长时期的不同而有所不同,均匀度指数(E)4个时期转双价棉与亲本常规棉间无显著差异。主成分分析结果表明,转双价棉苗期、花铃期与常规棉苗期、蕾期土壤微生物群落碳源利用类型相似,转双价棉蕾期与常规棉花铃期土壤微生物碳源利用类型相似。对不同碳源的分析结果表明土壤微生物利用的主要碳源为:糖类、氨基酸类、羧酸类、聚合物类。The aim was to study the effects of planting transgenic cotton on functional diversity in rhizosphere soil microbial community. With the method of Biolog, a comparative study was made on the utilization level of single carbon source by the microbes in the rhizosphere soils of transgenic Bt＋ CpTI cotton sGK321 and its parental isoline ＇Shiyuan 321＇ in different growth stages. The results showed that, the Average Well Color Development （A WCD） changed in the following variation： compared with the parental isoline, A WCD value of transgenic cotton at seedling stage and budding stage were significantly higher （P〈0.05） while flower and boll stage and boll-opening stage were significantly lower. The Shannon index （H） and Simpson＇s Dominance （D） of the soil microbial community varied with the growth stages, whereas the Substrate evenness （E） with no significant difference between transgenic cotton and its parental isoline in the four growth stages. Principal component analysis indicated that the carbon source utilization of soil microbial community was similar among transgenic cotton at seeding stage and flower and boll stage and parental isoline at seeding stage and budding stage, also the same finding among parental at flower and boll stage and transgenic at budding stage. The main carbon sources used by soil microbes were carbohydrates, amino acids, carboxylic acids, polymers.

关 键 词：转双价(Bt+CpTI)棉 土壤微生物 功能多样性 BIOLOG 主成分分析 

分 类 号：S154.36[农业科学—土壤学]