神经生长因子-明胶海绵复合体修复种植体周围骨缺损的实验研究  

Effect of nerve growth factor-gelatin sponge composite on peri-mplant osseointegration

作　　者：黄峰[1] 项立新[1] 徐国超[1] 

机构地区：[1]浙江医院口腔科,杭州310013

出　　处：《中华口腔医学杂志》2013年第1期23-26,共4页Chinese Journal of Stomatology

基　　金：浙江省医药卫生科技计划（2009A016）

摘　　要：目的观察外源性神经生长因子（nerve growth factor，NGF）-明胶海绵（gelatin sponge，GS）复合体（NGF．GS）对牙种植体周围骨缺损的修复作用。方法选择健康成年比格犬6只，分别拔除下颌双侧第一、二前磨牙，于远中拔牙窝行即刻种植，在种植体近中制作5mm×3mmX5him骨缺损。实验组植入NGF—GS（含NGF10斗g），对照组植入Gs，空白组不植入任何物质。分别于术后4、8和12周各处死动物2只并获取标本，制作种植体骨磨片，亚甲基蓝一酸性品红法染色，进行大体、放射学、组织学观察和骨计量学分析。结果术后4、8周3组种植体周骨缺损的新生骨密度低于周围正常骨组织。术后4周实验组种植体骨结合率[（57．7±6．4）％]显著高于对照组[（44．24-3．3）％]和空白组[（31．24-3．1）％]（P〈0．01），且对照组种植体骨结合率显著高于空白组（P〈0．01）；术后8周实验组种植体骨结合率[（94．84-7．7）％]略高于对照组[（83．04-4．1）％]和空白组[（86．44-6．3）％]，差异无统计学意义（P〉0．05）；术后12周各组骨密度与正常骨组织接近，种植体骨结合率无明显差异。结论NGF．GS在种植体骨结合早期能够增加口腔种植体周围新生骨小梁的面积，加速新生骨组织矿化，缩短骨结合时间，提高种植体骨结合率。Objective To observe the peri-irnplant osseointegration after the application of exogenous nerve growth factor （NGF） -gelatin sponge（GS） composite. Methods Six beagle dogs were used to establish hi-mandible simultaneous implant model after the first and second premolar extraction. Then the dental implants were implanted into the distal socket while the bone defect was made in the mesial socket of each mandible. Then the NGF-GS was implanted into the defects as NGF-GS group, the gelatin sponge alone was implanted as GS control and the control group was left empty, Two dogs were sacrificed each time at 4 weeks, 8 weeks and 12 weeks postoperatively. Specimens were subjected to general observation, radiography,bone histological and histomorphometric analysis for the new bone formation. The data were analyzed with SPSS 11.5 software package. Results The bone density in the defects around implants at 4 and 8 weeks postoperatively was lower than the normal bone. The bone-implant contact ratio in the NGF-GS group[ （57. 7±6.4）% ] was significantly higher than that in the GS control group and the control group [theratio were（44.2 ±3.3）% and（31.2 ± 3.1）%] （P 〈0.01） at 4 weeks postoperatively, the bone-implant contact ratio in the GS control group was also significantly higher than that in the control group （ P 〈 0. 01 ） at that time. The bone-implant contact ratio in the NGF-GS group [ （ 94. 8 ± 7. 7 ） % ] was slightly higher than that in the GS control group and the control group [ the ratio were （ 83.0 ± 4. 1 ） % and （ 86.4 ± 6. 3 ） % ] at 8 weeks postoperatively, but there was no statistical difference （ P 〉 0. 05 ）. The bone density in the defects around implants at 12 weeks was almost the same as the normal bone, there was no difference of the bone-implant contact ratio. Conclusions NGF-GS application could increase new bone formation, accelerate maturation of trabecular bone around the implants and shorten the period of osseointegration.

关 键 词：神经生长因子 明胶海绵 吸收性 牙种植体 骨结合 

分 类 号：R783.6[医药卫生—口腔医学]