痰液MicroRNAs检测在非小细胞肺癌诊断中的应用价值  

Diagnostic value of the detection of MicroRNAs in sputum of patients with non-small cell lung cancer

作　　者：杨小倩[1] 张义宏[1] 孙波[2] 邓鹏[3] 范骏[1] 沈景昊 朱广宇[1] 顾明芳[1] 

机构地区：[1]苏州市相城人民医院呼吸内科,江苏苏州215131 [2]苏州大学附属第一医院临床免疫研究所,江苏苏州215006 [3]苏州大学附属第一医院胸外科,江苏苏州215006

出　　处：《临床肺科杂志》2013年第2期226-229,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的检测肺癌患者痰中miRNA-21、miRNA-155、miRNA-137及let-7a的表达,探讨其在肺癌诊断中的价值。方法选取非小细胞肺癌(NSCLC)患者24例。肺良性疾病(OPD)患者24例。11例健康志愿者为对照。Real-time PCR检测痰中miRNA表达。结果 NSCLC患者痰中miRNA-21、miRNA-155表达均显著高于OPD患者及健康对照者,P均<0.01。NSCLC患者let-7a表达均显著低于OPD患者和健康对照者,P均<0.001。三组患者痰中miRNA-137表达无统计学差异,P均>0.05。miRNA-21、miRNA-155及let-7a从OPD中诊断NSCLC的ROC曲线下面积依次为0.863、0.840和0.887,P均<0.01。结论痰miRNAs可能成为肺癌诊断的一种新指标。Objective To detect the expression of miRNA-21, miRNA-155, miRNA-137 and let-7a in sputum of patients with non-small cell lung cancer （ NSCLC）, and to investigate their diagnostic significance for lung cancer. Method The study selected 24 pa- tients with NSCLC, 24 patients with other benign pulmonary diseases （OPD）, and 11 healthy people as the controls. The expression of miRNA in sputum was detected by Real-time PCR. Results The expression levels of miRNA-21 and miRNA-155 in sputum were signifi- cantly higher in the NSCLC group than in the OPD group and the control group. （ P 〈0.01 ）, but the expression of let-7a was just contrary to the above. There was no significantly difference of miRNA-137 expression among the three groups （ P 〉 0. 05 ）. Conclusion The measurement of altered miRNAs expression in sputum could be a useful noninvasive approach for the diagnosis of lung cancer.

关 键 词：痰 MICRORNA 肺癌 实时定量PCR 诊断 

分 类 号：R734.2[医药卫生—肿瘤]